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Gene Mapping, Cloning and Sequencing

The KOX zinc finger genes: Genome wide mapping of 368 ZNF PAC clones with zinc finger gene clusters predominantly in 23 chromosomal loci are confirmed by human sequences annotated in EnsEMBL

Rousseau-Merck M.F.a · Koczan D.b · Legrand I.a · Möller S.c · Autran S.a · Thiesen H-J.b

Author affiliations

aInserm U509, Institut Curie, Paris (France); bInstitute for Immunology and cProteome Center Rostock, Rostock (Germany)

Related Articles for ""

Cytogenet Genome Res 98:147–153 (2002)

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Article / Publication Details

First-Page Preview
Abstract of Gene Mapping, Cloning and Sequencing

Received: July 31, 2002
Accepted: December 03, 2002
Published online: April 14, 2003
Issue release date: 2002

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

The chromosome locations of 368 human Kruppel-type zinc finger (ZNF) PAC clones were physically mapped by FISH to human chromosomes in support of recent efforts of assigning KOX cDNAs (KOX1–KOX32) to zinc finger gene clusters. Recent mapping results were validated and confirmed by sequence comparisons to zinc finger gene sequences automatically annotated in EnsEMBL. In toto, 799 Kruppel-type zinc finger genes have been annotated in EnsEMBL of which 290 genes are found to encode KRAB domains. Sequence homologies of the zinc finger domains were used to establish phylogenic trees of KOX zinc finger genes as well as of all KRAB containing human zinc finger and KOX genes documenting the evolution of KRAB zinc finger genes late in primate evolution. A list of 368 assigned ZNF PAC clones is available under http://www.pzr.uni-rostock.de/supplements.   

© 2002 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Gene Mapping, Cloning and Sequencing

Received: July 31, 2002
Accepted: December 03, 2002
Published online: April 14, 2003
Issue release date: 2002

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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