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Table of Contents
Vol. 77, No. 5, 2003
Issue release date: June 2003
Section title: Regulation of Reproductive Hormones
Neuroendocrinology 2003;77:314–323
(DOI:10.1159/000070897)

Evidence for a GABAergic System in Rodent and Human Testis: Local GABA Production and GABA Receptors

Geigerseder C.a · Doepner R.a · Thalhammer A.a · Frungieri M.B.a,c · Gamel-Didelon K.a · Calandra R.S.c · Köhn F.M.b · Mayerhofer A.a
aAnatomisches Institut der Universität München, München, Deutschland; bKlinik und Poliklinik für Dermatologie und Allergologie der Technischen Universität München, München, Deutschland; cInstituto de Biología y Medicina Experimental, Facultad de Ciencias Exactas, UNLP, Buenos Aires, Argentina

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Article / Publication Details

First-Page Preview
Abstract of Regulation of Reproductive Hormones

Received: September 26, 2002
Accepted: January 23, 2003
Published online: June 13, 2003
Issue release date: June 2003

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 2

ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)

For additional information: http://www.karger.com/NEN

Abstract

The major neurotransmitter of the central nervous system, gamma-aminobutyric acid (GABA), exerts its actions through GABAA, GABAB and GABAC receptors. GABA and GABA receptors are, however, also present in several non-neural tissues, including the endocrine organs pituitary, pancreas and testis. In the case of the rat testis, GABA appears to be linked to the regulation of steroid synthesis by Leydig cells via GABAA receptors, but neither testicular sources of GABA, nor the precise nature of testicular GABA receptors are fully known. We examined these points in rat, mouse, hamster and human testicular samples. RT-PCR followed by sequencing showed that the GABA-synthesizing enzymes glutamate decarboxylase (GAD) 65 and/or GAD67, as well as the vesicular GABA transporter vesicular inhibitory amino acid transporter (VIAAT/VGAT) are expressed. Testicular GAD in the rat was shown to be functionally active by using a GAD assay, and Western blot analysis confirmed the presence of GAD65 and GAD67. Interstitial cells, most of which are Leydig cells according to their location and morphological characteristics, showed positive immunoreaction for GAD and VIAAT/VGAT proteins. In addition, several GABAA receptor subunits (α1–3, β1–3, γ1–3), as well as GABAB receptor subunits R1 and R2, were detected by RT-PCR. Western blot analysis confirmed the results for GABAA receptor subunits β2/3 in the rat, and immunohistochemistry identified interstitial Leydig cells to possess immunoreactive GABAA receptor subunits β2/3 and α1. The presence of GABAA receptor subunit α1 mRNA in interstitial cells of the rat testis was further shown after laser microdissection followed by RT-PCR analysis. In summary, these results describe molecular details of the components of an intratesticular GABAergic system expressed in the endocrine compartment of rodent and human testes. While the physiological significance of this peripheral neuroendocrine system conserved throughout species remains to be elucidated, its mere presence in humans suggests the possibility that clinically used drugs might be able to interfere with testicular function.

© 2003 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Regulation of Reproductive Hormones

Received: September 26, 2002
Accepted: January 23, 2003
Published online: June 13, 2003
Issue release date: June 2003

Number of Print Pages: 10
Number of Figures: 5
Number of Tables: 2

ISSN: 0028-3835 (Print)
eISSN: 1423-0194 (Online)

For additional information: http://www.karger.com/NEN


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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