The general transcriptional repressor Tup1p requires the cyclin/cyclin-dependent kinase pair Srb11p/Srb10p in the holoenzyme of transcription. We used the split-ubiquitin system to demonstrate that Tup1p interacts with Srb11p in vivo. We confirmed our observation in vitro with the help of purified proteins, and we compared the de-repression effect of deleting TUP1, SRB10, and SRB11 on different promoters. We propose that Tup1p targets the cyclin Srb11p to affect the cyclin-dependent kinase Srb10p.
© 2003 S. Karger AG, Basel
Department of Microbiology, Faculty of Medicine, National University of Singapore
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Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 0, Number of References : 39
Journal of Molecular Microbiology and Biotechnology
Founded 1999 by M.H. Saier, Jr.
Vol. 5, No. 4, Year 2003 (Cover Date: Released July 2003)
Journal Editor: M.H. Saier, Jr., La Jolla, Calif.
ISSN: 1464–1801 (print), 1660–2412 (Online)
For additional information: http://www.karger.com/mmb
Article / Publication Details
Published online: 7/16/2003
Issue release date: July 2003
Number of Print Pages: 7
Number of Figures: 4
Number of Tables: 0
ISSN: 1464-1801 (Print)
eISSN: 1660-2412 (Online)
For additional information: http://www.karger.com/MMB
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