The general transcriptional repressor Tup1p requires the cyclin/cyclin-dependent kinase pair Srb11p/Srb10p in the holoenzyme of transcription. We used the split-ubiquitin system to demonstrate that Tup1p interacts with Srb11p in vivo. We confirmed our observation in vitro with the help of purified proteins, and we compared the de-repression effect of deleting TUP1, SRB10, and SRB11 on different promoters. We propose that Tup1p targets the cyclin Srb11p to affect the cyclin-dependent kinase Srb10p.
Department of Microbiology, Faculty of Medicine, National University of Singapore
Block MD4, 5 Science Drive 2
Singapore 117597 (Singapore)
Tel. +65 6874 3499, Fax +65 6776 6872, E-Mail firstname.lastname@example.org
Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 0, Number of References : 39
Journal of Molecular Microbiology and Biotechnology
Founded 1999 by M.H. Saier, Jr.
Vol. 5, No. 4, Year 2003 (Cover Date: Released July 2003)
Journal Editor: M.H. Saier, Jr., La Jolla, Calif.
ISSN: 1464–1801 (print), 1660–2412 (Online)
For additional information: http://www.karger.com/mmb
Article / Publication Details
Copyright / Drug Dosage
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.