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Vol. 35, No. 4, 2003
Issue release date: July–August 2003
Section title: Original Paper
Ophthalmic Res 2003;35:199–207
(DOI:10.1159/000071171)

Expression of Tissue Inhibitor of Metalloproteinase-4 in Normal Human Corneal Cells and Experimental Corneal Neovascularization

Ma D.H.-K. · Zhang F. · Shi W. · Yao J.-Y. · Hsiao C.-H. · Wu H.C. · Kim W.-S. · Hao Y.-X. · Hwang D.G. · Chen J.-K. · Tsai R.J.-F.
aDepartment of Ophthalmology, Chang-Gung Memorial Hospital, Taoyuan, Taiwan; bOcular Cell and Gene Therapy Laboratory, Department of Ophthalmology, University of California, San Francisco, Calif., USA; cExelixis, South San Francisco, Calif., USA; dMaryknoll Hospital, and ePusan National University, Pusan, Korea; fDepartment of Physiology, Chang-Gung University, Taoyuan, Taiwan

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/13/2002
Accepted: 3/4/2003
Published online: 6/20/2003

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 0

ISSN: 0030-3747 (Print)
eISSN: 1423-0259 (Online)

For additional information: http://www.karger.com/ORE

Abstract

Purpose: To study the expression of TIMP-4 in cultured corneal cells and in corneal neovascularization. Methods: Human limbo-corneal epithelial cells, fibroblasts, and endothelial cells were cultured in serum-free, PMA- or basic fibroblast growth factor (bFGF)-treated condition. Neovascularization in rat cornea was induced by suturing. The expression of TIMP-4 was examined by immunohistochemistry, Western blot and RT-PCR. Results: TIMP-4 was constitutively expressed in cultured human corneal cells. The expression was only mildly enhanced after mitogen treatment. TIMP-4 immunoreactivity was predominantly expressed in normal rat corneal epithelium, and also in ingrowing blood vessels following suturing, which persisted up to day 28. Increased staining in corneal epithelium and blood vessels were also noted in vascularized human corneas. Conclusions: TIMP-4 is expressed in the cornea, which may play a role in modulating extracellular matrix remodeling associated with corneal wound healing and angiogenesis.


  

Author Contacts

Dr. David Hui-Kang Ma
Department of Ophthalmology
Chang-Gung Memorial Hospital, No. 5, Fu-Shin Street
Kuei-Shan Township, Taoyuan County 333 (Taiwan)
Tel. +886 3 3281200, ext. 8666, Fax +886 3 3287798, E-Mail davidhkma@yahoo.com

  

Article Information

Received: July 13, 2002
Accepted after revision: March 4, 2003
Number of Print Pages : 9
Number of Figures : 5, Number of Tables : 0, Number of References : 40

  

Publication Details

Ophthalmic Research (Journal for Research in Experimental and Clinical Ophthalmology)
Founded 1970 by O. Hockwin, Bonn, G. Naumann, Hamburg and D.F. Cole, London

Vol. 35, No. 4, Year 2003 (Cover Date: July-August 2003)

Journal Editor: Gijs F.J.M. Vrensen, Zeist
ISSN: 0030–3747 (print), 1423–0259 (Online)

For additional information: http://www.karger.com/ore


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/13/2002
Accepted: 3/4/2003
Published online: 6/20/2003

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 0

ISSN: 0030-3747 (Print)
eISSN: 1423-0259 (Online)

For additional information: http://www.karger.com/ORE


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