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Calibrated Automated Thrombin Generation in Frozen-Thawed Platelet-Rich Plasma to Detect Hypercoagulability

Regnault V.a · Béguin S.c · Lecompte T.a,b

Author affiliations

aINSERM ERIT-M 0323 et EA 3452, Faculté de Médecine, Université Henri Poincaré, et bHématologie Biologique, CHU de Nancy, Nancy, France; cSynapse BV, Cardiovascular Research Institute, University of Maastricht, Maastricht, TheNetherlands

Corresponding Author

Véronique Regnault

INSERM ERIT-M 0323 ‘Nouvelles approches anti-thrombotiques’ EA 3452

Faculté de Médecine, Université Henri Poincaré, Boite Postale 184

FR–54505 Vandoeuvre-lès-Nancy (France)

Tel. +33 3 83 68 34 70, Fax +33 3 83 38 34 79, E-mail regnault@hemato.u-nancy.fr

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Pathophysiol Haemost Thromb 2003;33:23–29

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Abstract

To enhance the practical applicability of the calibrated automated thrombogram (CAT) we investigated whether frozen-thawed platelet-rich plasma (ft-PRP) can be used to assess the function of the protein C inhibitory pathway, while preserving the natural phospholipid composition. Recalcified ft-PRP triggered with 0.5 pM recombinant human tissue factor shows a median thrombin potential of 1,779 nM·min, against 1,576 nM·min for fresh PRP. To obtain ∼70% inhibition, 6.7 nM activated protein C (APC) has to be added, instead of 25 nM in fresh PRP; so the relative APC resistance of PRP appears to depend upon the presence of intact platelets. Factor VIII, added to normal ft-PRP to obtain a concentration of 3.3 U/ml, increases the thrombin potential in the presence of APC 1.5-fold, from 524 to 808 nM·min, in keeping with previously published increases in thrombotic risk in patients with high factor VIII levels. We conclude that thrombography in ft-PRP, with and without added APC, can be used to assess known risk factors for thrombosis, which allows the design of large clinical studies aimed at proving the relationship between thrombin potential and clinical outcome.

© 2003 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: August 08, 2003
Issue release date: June 2003

Number of Print Pages: 7
Number of Figures: 6
Number of Tables: 0

ISSN: 1424-8832 (Print)
eISSN: 1424-8840 (Online)

For additional information: http://www.karger.com/PHT


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