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Vol. 40, No. 3, 2003
Issue release date: May–June 2003
Section title: Research Paper
J Vasc Res 2003;40:266–275
(DOI:10.1159/000071890)

Beta-Galactosidase-Tagged Adventitial Myofibroblasts Tracked to the Neointima in Healing Rat Vein Grafts

Tomas J.J. · Stark V.E. · Kim J.L. · Wolff R.A. · Hullett D.A. · Warner T.F. · Hoch J.R.
Departments of aSurgery and bPathology, University of Wisconsin School of Medicine, Madison, Wisc., USA

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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 4/12/2002
Accepted: 12/3/2002
Published online: 8/8/2003

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR

Abstract

Objective: Myofibroblasts are present transiently in normal healing wounds. However, they have been found to persist in the stroma of neoplasms, fibrotic conditions and other pathological settings. In rat vein grafts, we have observed the prolonged presence of myofibroblasts. Our aim was to determine the origin of myofibroblasts in vein grafts. Methods: Epigastric vein to femoral artery grafts were microsurgically placed in male Lewis rats and harvested. Neointimal development, cellular death and proliferation, and cell phenotypes were analyzed using immunohistochemistry and light and electron microscopy. To follow cellular movement in the vessel wall, vein grafts were transfected with replication-defective adenovirus containing the gene encoding β-galactosidase (n = 50), and harvested at 1, 2, 3, 4, 5, 6, 7, 14 and 28 days. Grafts were analyzed after X-gal staining. Results: Myofibroblasts were detected in the outer adventitia at 4 days, in the media at 1 week and in the developing neointima at 2 weeks. Cells tagged using adenoviral β-galactosidase demonstrated adventitia to neointima cell migration. Conclusions: Although there may be other sources of myofibroblasts in this model, the adventitia has been shown to be an origin of myofibroblasts which subsequently migrate through the vessel wall to the neointima during graft remodeling and contribute to neointimal formation.


  

Author Contacts

Dr. John R. Hoch
University of Wisconsin Hospitals and Clinics
G5/325 CSC, 600 Highland Avenue
Madison, WI 53792 (USA)
Tel. +1 608 263 1388, Fax +1 608 263 7652, E-Mail hoch@surgery.wisc.edu

  

Article Information

Received: April 12, 2002
Accepted after revision: December 3, 2002
Number of Print Pages : 10
Number of Figures : 6, Number of Tables : 1, Number of References : 35

  

Publication Details

Journal of Vascular Research (Incorporating International Journal of Microcirculation)
Founded 1964 as Angiologica by M. Comèl and L. Laszt (1964–1973) continued as Blood Vessels by J.A. Bevan (1974–1991)
Official Journal of the European Society for Microcirculation

Vol. 40, No. 3, Year 2003 (Cover Date: May-June 2003)

Journal Editor: U. Pohl, Munich
ISSN: 1018–1172 (print), 1423–0135 (Online)

For additional information: http://www.karger.com/jvr


Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 4/12/2002
Accepted: 12/3/2002
Published online: 8/8/2003

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR


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