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Vol. 40, No. 4, 2003
Issue release date: July–August 2003
Section title: Research Paper
J Vasc Res 2003;40:351–358
(DOI:10.1159/000072699)

In vitro Study of Starling’s Hypothesis in a Cultured Monolayer of Bovine Aortic Endothelial Cells

Pang Z. · Tarbell J.M.
Biomolecular Transport Dynamics Laboratory, Departments of Chemical Engineering and Bioengineering, The Pennsylvania State University, University Park, Pa., USA

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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 12/16/2002
Accepted: 3/6/2003
Published online: 9/26/2003

Number of Print Pages: 8
Number of Figures: 5
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR

Abstract

Starling’s hypothesis that fluid movement across the microvascular wall is determined by the transmural differences in hydrostatic and osmotic pressures was tested using an in vitro model comprised of bovine aortic endothelial cells grown on a porous support. In all experiments, a 1% bovine serum albumin (BSA) solution was maintained in the abluminal reservoir and the luminal reservoir contained either a 1 or a 5.5% BSA solution. The global osmotic pressure difference across the endothelial layers was thus either 0 or 20.3 cm H2O. When the luminal concentration of BSA was changed from 1 to 5.5% at a hydrostatic pressure differential of 5, 10 or 20 cm H2O, no reverse flow (in the reabsorption direction) was observed even though the hydrostatic pressure differential was far below the global osmotic pressure differential. In another case, the hydrostatic pressure differential was dropped quickly from 20 to 5 cm H2O, while a constant osmotic pressure differential was maintained by 5.5% BSA in the luminal reservoir. A strong transient reabsorption flow was observed over a 30-second period which diminished to undetectable levels within 2.5 min; then a sustained steady-state filtration flow was observed after 20 min. These in vitro experiments support other studies in capillaries showing transient reabsorption that decays to steady-state filtration at longer times.


  

Author Contacts

Dr. John M. Tarbell
Steinman Hall, Department of Biomedical Engineering
City University of New York
New York, NY 10031 (USA)
Tel. +1 212 650 6841, Fax +1 212 650 5768, E-Mail tarbell@ccny.cuny.edu

  

Article Information

Received: December 16, 2002
Accepted after revision: March 6, 2003
Published online: July 29, 2003
Number of Print Pages : 8
Number of Figures : 5, Number of Tables : 1, Number of References : 21

  

Publication Details

Journal of Vascular Research (Incorporating International Journal of Microcirculation)
Founded 1964 as Angiologica by M. Comèl and L. Laszt (1964–1973) continued as Blood Vessels by J.A. Bevan (1974–1991)
Official Journal of the European Society for Microcirculation

Vol. 40, No. 4, Year 2003 (Cover Date: July-August 2003)

Journal Editor: U. Pohl, Munich
ISSN: 1018–1172 (print), 1423–0135 (Online)

For additional information: http://www.karger.com/jvr


Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 12/16/2002
Accepted: 3/6/2003
Published online: 9/26/2003

Number of Print Pages: 8
Number of Figures: 5
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR


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