Visualization of Leukocyte Transendothelial and Interstitial Migration Using Reflected Light Oblique Transillumination in Intravital Video MicroscopyMempel T.R. · Moser C. · Hutter J. · Kuebler W.M. · Krombach F.
Institute for Surgical Research, University of Munich, Munich, Germany
Dynamic visualization of the intravascular events leading to the extravasation of leukocytes into tissues by intravital microscopy has significantly expanded our understanding of the underlying molecular processes. In contrast, the detailed observation of leukocyte transendothelial and interstitial migration in vivo has been hampered by the poor image contrast of cells within turbid media that is obtainable by conventional brightfield microscopy. Here we present a microscopic method, termed reflected light oblique transillumination microscopy, that makes use of the optical interference phenomena generated by oblique transillumination to visualize subtle gradients of refractive indices within tissues for enhanced image contrast. Using the mouse cremaster muscle, we demonstrate that this technique makes possible the reliable quantification of extravasated leukocytes as well as the characterization of morphological phenomena of leukocyte transendothelial and interstitial migration.
Dr. Fritz Krombach
Institute for Surgical Research, University of Munich
Marchioninistrasse 27, DE–81377 Munich (Germany)
Tel. +49 89 7095 4359, Fax +49 89 7095 4353
Received: June 15, 2003
Accepted after revision: September 5, 2003
Published online: October 3, 2003
Number of Print Pages : 7
Number of Figures : 4, Number of Tables : 0, Number of References : 20
Additional supplemental material is available online.
Journal of Vascular Research (Incorporating International Journal of Microcirculation)
Founded 1964 as Angiologica by M. Comèl and L. Laszt (1964–1973) continued as Blood Vessels by J.A. Bevan (1974–1991)
Official Journal of the European Society for Microcirculation
Vol. 40, No. 5, Year 2003 (Cover Date: September-October 2003)
Journal Editor: U. Pohl, Munich
ISSN: 1018–1172 (Print), eISSN: 1423–0135 (Online)
For additional information: http://www.karger.com/jvr