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Vol. 12, No. 6, 2003
Issue release date: November–December 2003
Section title: Original Paper
Neurosignals 2003;12:283–291
(DOI:10.1159/000075310)

Translational Activity of N-Methyl-D-Aspartate Receptor Subunit NR1 mRNA in PC12 Cells

Awobuluyi M.a · Vazhappilly R.d · Sucher N.J.b,c,d
aDepartment of Neurobiology, Harvard Medical School, Boston, Mass., USA; bMolecular Neuroscience Center, cBiotechnology Research Institute and dDepartment of Biology, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 11/25/2002
Accepted: 4/8/2003
Published online: 1/28/2004
Issue release date: November–December 2003

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 0

ISSN: 1424-862X (Print)
eISSN: 1424-8638 (Online)

For additional information: http://www.karger.com/NSG

Abstract

PC12 cells contain NR1 mRNA but lack significant expression of NR1 protein suggesting translational or posttranslational regulation. Translational activity of NR1 mRNA in PC12 cells was examined by sucrose gradient fractionation and by heterologous luciferase NR1 gene expression studies. The cosedimentation and association of NR1 mRNA with large polyribosomes (polysomes) confirmed the translatability of NR1 message in PC12 cells. Possible initiation and/or elongation defects during the translation of NR1 mRNAs were investigated by cyclohexamide treatment. The marked decline in the number of ribosomes associated with NR1 mRNA after prolonged exposure to cyclohexamide suggested that initiation was limiting translation of NR1 mRNA in PC12 cells. Consequently, the effect of the 5′ and 3′ untranslated regions (UTRs) on translation was examined using fusion constructs consisting of the luciferase coding region fused to either or both the 5′ UTR and 3′ UTR of NR1. The transfection of PC12 cells with the luciferase NR1-UTR fusion constructs revealed that the 3′ UTR of NR1 had a significant inhibitory effect on luciferase expression. In contrast, the 5′ UTR of NR1 had no inhibitory effect on mRNA translation in PC12 cells. The results from this study indicate that the translation of NR1 mRNA in PC12 cells may be impeded at initiation and this inhibition may be regulated at least in part through the 3′ UTR of NR1.

© 2003 S. Karger AG, Basel


  

Author Contacts

Nikolaus J. Sucher
Department of Biology, Hong Kong University of Science and Technology
Clear Water Bay, Kowloon, Hong Kong SAR (China)
Tel. +86 852 2358 7306, Fax +86 852 2358 1559
E-Mail sucher@ust.hk

  

Article Information

Received: November 25, 2002
Accepted after revision: April 8, 2003
Number of Print Pages : 9
Number of Figures : 5, Number of Tables : 0, Number of References : 39

  

Publication Details

Neurosignals
Founded 1992 as ‘Biological Signals’ by S.F. Pang (1992–2001) continued as ‘Biological Signals and Receptors’ (1999–2001)

Vol. 12, No. 6, Year 2003 (Cover Date: November-December 2003)

Journal Editor: Nancy Y. Ip, Hong Kong
ISSN: 1424–862X (print), 1424–8638 (Online)

For additional information: http://www.karger.com/journals/nsg


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 11/25/2002
Accepted: 4/8/2003
Published online: 1/28/2004
Issue release date: November–December 2003

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 0

ISSN: 1424-862X (Print)
eISSN: 1424-8638 (Online)

For additional information: http://www.karger.com/NSG


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