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Table of Contents
Vol. 102, No. 1-4, 2003
Issue release date: 2003
Section title: Paper
Cytogenet Genome Res 102:249–253 (2003)
(DOI:10.1159/000075757)

Isolation and chromosomal assignment of canine genomic BAC clones representing 25 cancer-related genes

Thomas R.a,b · Bridge W.a · Benke K.a · Breen M.a,b
aCentre for Preventive Medicine, Animal Health Trust, Lanwades Park, Kentford, Newmarket, Suffolk (UK); bDepartment of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh NC (USA)

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: March 01, 2004
Issue release date: 2003

Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

An extensive number of genes have been implicated in the initiation and progression of human cancers, aiding our understanding of the genetic aetiology of this highly heterogeneous disease. In order to facilitate extrapolation of such information between species, we have isolated and physically mapped the canine orthologues of 25 well-characterised human cancer-related genes. The identity of PCR products representing each canine gene marker was first confirmed by DNA sequencing analysis. Each product was then radiolabelled and used to screen a genomic BAC library for the domestic dog. The chromosomal location of each positive clone in the canine karyotype was determined by fluorescence in situ hybridisation (FISH) onto canine metaphase preparations. Of the 25 genes, the FISH localisation of 21 correlated fully with that expected on the basis of known regions of conserved synteny between the human and canine genomes. Three correlated less closely, and the chromosomal location of the remaining marker showed no apparent correlation with current comparative mapping data. In addition to generating useful comparative mapping information, this panel of markers will act as a valuable resource for detailed study of candidate genes likely to be involved in tumourigenesis, and also forms the basis of a canine cancer-gene genomic microarray currently being developed for the study of unbalanced genomic aberrations in canine tumours.   

© 2003 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: March 01, 2004
Issue release date: 2003

Number of Print Pages: 5
Number of Figures: 0
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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