Background and Aims: Inoculation of Helicobacter felis into the murine stomach has been reported to induce chronic gastric inflammation and may be a model of Helicobacter pylori-induced chronic gastritis. In this study, to characterize H. felis-induced gastritis, the gastric production of interleukin-1β (IL-1β) and hepatocyte growth factor (HGF) was measured in mice. Methods: Gastric mucosal lesions were induced in H. felis-infected BALB/c mice by water-immersion stress. The severity score of gastric erosions per stomach was measured as the sum of the length of erosions. Gene expression of IL-1β and HGF were analyzed by Northern blot analysis and production of HGF was examined using the enzyme immunoassay method. Results: Water-immersion stress induced gastric mucosal lesions accompanied by increased expression of IL-1β mRNA. H. felis infection evoked enhanced expression of IL-1β and HGF genes. When H. felis-infected mice were stressed by water immersion, the mucosal lesions were more severe than those in non-infected mice. Moreover, IL-1β gene expression as well as HGF production was further increased. Conclusions: Although H. felis inoculation did not cause gastric mucosal erosions by itself, it augmented the stress-induced erosions.
Tsutomu Chiba, MD
Department of Gastroenterology and Hepatology
Kyoto University Graduate School of Medicine
54, Kawahara-cho, Shougoin, Sakyo-ku, Kyoto 606-01 (Japan)
Tel. +81 75 751 4319, Fax +81 75 751 4303
Received: Received: September 1, 1997
Accepted: June 13, 1998
Number of Print Pages : 7
Number of Figures : 8, Number of Tables : 1, Number of References : 23
Digestion (International Journal of Gastroenterology)
Founded as Archiv für Verdauungskrankheiten, 1895; continued as Gastroenterologia, 1939-1967
Vol. 60, No. 1, Year 1999 (Cover Date: January-February 1999)
Journal Editor: R. Arnold, Marburg
ISSN: 0012–2823 (print), 1421–9867 (Online)
For additional information: http://www.karger.com/journals/dig
Article / Publication Details
Published online: 1/18/1999
Issue release date: January–February 1999
Number of Print Pages: 7
Number of Figures: 8
Number of Tables: 1
ISSN: 0012-2823 (Print)
eISSN: 1421-9867 (Online)
For additional information: http://www.karger.com/DIG
Copyright / Drug Dosage
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.