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Vol. 11, No. 2, 2004
Issue release date: March–April 2004
Section title: Original Paper
J Biomed Sci 2004;11:186–199
(DOI:10.1159/000076031)

Glossogyne tenuifolia Acts to Inhibit Inflammatory Mediator Production in a Macrophage Cell Line by Downregulating LPS-Induced NF-κB

Wu M.-J.a · Wang L.a · Ding H.-Y.b · Weng C.-Y.a · Yen J.-H.c
Departments of aBiotechnology and bCosmetic Science, Chia-Nan University of Pharmacy and Science, and cScinoPharm Biotech Ltd, Tainan Science-Based Industrial Park, Tainan, Taiwan, ROC

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/6/2003
Accepted: 10/3/2003
Published online: 2/19/2004
Issue release date: March–April 2004

Number of Print Pages: 14
Number of Figures: 10
Number of Tables: 0

ISSN: 1021-7770 (Print)
eISSN: 1423-0127 (Online)

For additional information: http://www.karger.com/JBS

Abstract

Glossogyne tenuifolia (hsiang-ju) (GT) is a traditional antipyretic herb used in Chinese medicine; however, no information is available to explain its action. The objective of this research was to elucidate the molecular pharmacological activity and the effective components in the ethanol extract of GT. We found that GT had potent anti-inflammatory effects on the lipopolysaccharide (LPS)-activated murine macrophages, RAW264.7. GT downregulated LPS-induced expression of inducible nitric oxide synthase (iNOS) by blocking its transcription. GT also caused a dose-dependent inhibition of the release of prostaglandin E2 by repressing the promoter activity of the inducible cyclooxygenase (COX-2) gene. Moreover, GT exerted a dose-dependent inhibition of the LPS-stimulated release of the proinflammatory cytokines, TNF-α, IL-1β, IL-6, and IL-12. To determine the mechanism by which GT inhibits LPS signaling, we focused on nuclear factor-ĸB (NF-ĸB) activation. Western blot analysis revealed that GT abolished LPS-induced inhibitor-ĸB phosphorylation. The electrophoretic mobility shift assay demonstrated that GT abolished LPS-mediated ĸB DNA binding activity. Moreover, macrophages were transfected with a vector coding for the luciferase reporter gene under the control of NF-ĸB cis-acting elements, and the transfected macrophages showed that the LPS-stimulated luciferase activity was GT-sensitive. These results suggest that GT attenuates inflammatory mediator synthesis of activated macrophages through an NF-ĸB-dependent pathway. The active components of GT were identified as oleanolic acid and luteolin-7-glucoside. Both of these compounds inhibited LPS-stimulated inflammatory mediator production and NF-ĸB activation. We conclude that GT inhibits NF-ĸB-mediated gene expression and downregulates inflammatory mediator production in murine macrophages.

© 2004 National Science Council, ROC and S. Karger AG, Basel


  

Author Contacts

Dr. Ming-Jiuan Wu
Department of Biotechnology
Chia-Nan University of Pharmacy and Science
Tainan 717, Taiwan (ROC)
Tel. +886 6 266 4911, ext. 220, Fax +886 6 266 6411, E-Mail imwu@mail.chna.edu.tw

  

Article Information

Received: June 6, 2003
Accepted: October 3, 2003
Number of Print Pages : 14
Number of Figures : 10, Number of Tables : 0, Number of References : 30

  

Publication Details

Journal of Biomedical Science (Sponsored by the National Science Council, Taipei)

Vol. 11, No. 2, Year 2004 (Cover Date: March-April 2004)

Journal Editor: S.H.H.Chan, Kaohsiung
ISSN: 1021–7770 (print), 1423–0127 (Online)

For additional information: http://www.karger.com/journals/jbs


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/6/2003
Accepted: 10/3/2003
Published online: 2/19/2004
Issue release date: March–April 2004

Number of Print Pages: 14
Number of Figures: 10
Number of Tables: 0

ISSN: 1021-7770 (Print)
eISSN: 1423-0127 (Online)

For additional information: http://www.karger.com/JBS


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