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Vol. 41, No. 1, 2004
Issue release date: January–February 2004
Section title: Research Paper
J Vasc Res 2004;41:28–37
(DOI:10.1159/000076126)

ICAM-3 Activation Modulates Cell-Cell Contacts of Human Bone Marrow Endothelial Cells

van Buul J.D. · Mul F.P.J. · van der Schoot C.E. · Hordijk P.L.
aDepartment of Experimental Immunohematology, Sanquin Research at CLB and the Landsteiner Laboratorium, University of Amsterdam, bDepartment of Hematology, Academic Medical Center, Amsterdam, The Netherlands

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Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 7/14/2003
Accepted: 10/23/2003
Published online: 2/20/2004

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 0

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR

Abstract

The Ig-like cell adhesion molecule ICAM-3 is mainly expressed on human leukocytes and is involved in cell-cell interactions. Its expression on endothelium is observed during disorders such as Crohn’s disease and in solid tumors. We found low but detectable expression of ICAM-3 on VE-cadherin-expressing cells from primary human bone marrow aspirates, i.e. endothelial cells, and on primary human endothelial cells from cord blood. Also, immortalized human umbilical cord endothelial cells and human bone marrow endothelial cells showed ICAM-3 expression. However, its function on human endothelium is not known. Surprisingly, activation of endothelial ICAM-3 by crosslinking with specific antibodies resulted in a drop in the electrical resistance of bone marrow endothelial monolayers. In line with this, immunocytochemical analysis showed a loss of endothelial cell-cell contacts after ICAM-3 crosslinking in HBMEC. Detailed biochemical analysis showed an association of moesin and in a later stage ezrin with ICAM-3 upon crosslinking in HBMEC. Moreover, ICAM-3 crosslinking induced the production of reactive oxygen species (ROS), which are known to be involved in the control of endothelial cell-cell contacts. In conclusion, we showed that ICAM-3 is expressed on human bone marrow endothelial cells and controls endothelial integrity via ROS-dependent signaling.


  

Author Contacts

Dr. Peter L. Hordijk
Department of Experimental Immunohematology, Sanquin Research at CLB
Plesmanlaan 125
NL–1066 CX, Amsterdam (The Netherlands)
Tel. +31 20 5123347, Fax +31 20 5123474, E-Mail p.hordijk@sanquin.nl

  

Article Information

Received: July 14, 2003
Accepted: October 23, 2003
Published online: January 12, 2004
Number of Print Pages : 10
Number of Figures : 6, Number of Tables : 0, Number of References : 25

  

Publication Details

Journal of Vascular Research (Incorporating International Journal of Microcirculation)
Founded 1964 as Angiologica by M. Comèl and L. Laszt (1964–1973) continued as Blood Vessels by J.A. Bevan (1974–1991)
Official Journal of the European Society for Microcirculation

Vol. 41, No. 1, Year 2004 (Cover Date: January-February 2004)

Journal Editor: U. Pohl, Munich
ISSN: 1018–1172 (print), 1423–0135 (Online)

For additional information: http://www.karger.com/jvr


Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 7/14/2003
Accepted: 10/23/2003
Published online: 2/20/2004

Number of Print Pages: 10
Number of Figures: 6
Number of Tables: 0

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR


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