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Vol. 218, No. 2, 2004
Issue release date: March–April 2004
Section title: Original Paper
Ophthalmologica 2004;218:136–140
(DOI:10.1159/000076150)

Thermomechanical Behavior of Collagen-Cross-Linked Porcine Cornea

Spoerl E. · Wollensak G. · Dittert D.-D. · Seiler T.
Departments of aOphthalmology and bPathology, Universitätsklinikum CGC, Dresden, Germany; cInstitut für Refraktive und okuloplastische Chirurgie (IROC), Zürich, Switzerland

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 10/9/2003
Published online: 3/4/2004

Number of Print Pages: 5
Number of Figures: 3
Number of Tables: 0

ISSN: 0030-3755 (Print)
eISSN: 1423-0267 (Online)

For additional information: http://www.karger.com/OPH

Abstract

Purpose: Collagen cross-linking using combined riboflavin/UVA treatment has been shown to increase the biomechanical rigidity of the cornea and has been used successfully for the treatment of progressive keratoconus. From morphological and biochemical investigations, a different degree of cross-linking for the anterior and posterior stroma by the treatment is suggested. The present study was undertaken to better evaluate this effect by testing the thermomechanical behavior. Methods: Ten 10 × 5 mm corneal strips from porcine cadaver eyes enucleated within 5 h post mortem were cross-linked using the photosensitizer riboflavin and UVA irradiation (370 nm, irradiance = 3 mW/cm2) for 30 min and compared to ten untreated corneal strips and ten corneal strips cross-linked with 0.1% glutaraldehyde. The temperature in a water bath was raised from 60 to 95°C with temperature increments of 1°C per minute. The hydrothermal shrinkage of the corneal strips was measured in 2.5°C steps using a micrometer. In addition, six 10-mm whole corneal buttons were cross-linked with riboflavin/UVA and immersed into water at 70 or 75°C. Results: The maximal hydrothermal shrinkage for the untreated control specimens and the posterior portion of the riboflavin/UVA-treated corneas was at 70°C, for the anterior portion of the cornea cross-linked by riboflavin/UVA at 75°C and for glutaraldehyde-cross-linked cornea at 90°C. In the cross-linked corneal buttons, a typical mushroom-like shape was observed at 70°C and a cylinder shape at 75°C. Conclusions: The different degree of collagen cross-linking in the corneal stroma after riboflavin/UVA treatment is reflected by the differences in the maximal shrinkage temperature of the anterior and posterior portion. Therefore, in the corneas cross-linked with riboflavin/UVA a higher shrinkage temperature was observed for the anterior portion of the cornea (75°C) compared to the posterior stroma (70°C) due to the higher degree of cross-linking of the anterior stroma. The anterior localization of the cross-linking effect is advantageous for the endothelium and for the preservation of the anterior corneal curvature.


  

Author Contacts

PD Dr. Eberhard Spoerl
Department of Ophthalmology, Universitätsklinikum CGC
Fetscherstrasse 74, DE–01307 Dresden (Germany)
Tel. +49 351 458 3763, Fax +49 351 458 4335
E-Mail eberhard.spoerl@uniklinikum-dresden.de

  

Article Information

Received: June 6, 2003
Accepted after revision: October 9, 2003
Number of Print Pages : 5
Number of Figures : 3, Number of Tables : 0, Number of References : 13

  

Publication Details

Ophthalmologica (International Journal of Ophthalmology)
Founded 1899 as ‘Zeitschrift für Augenheilkunde’ by H. Kuhnt and J. von Michel

Vol. 218, No. 2, Year 2004 (Cover Date: March-April 2004)

Journal Editor: Ch. Ohrloff, Frankfurt a.M.
ISSN: 0030–3755 (print), 1423–0267 (Online)

For additional information: http://www.karger.com/oph


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 10/9/2003
Published online: 3/4/2004

Number of Print Pages: 5
Number of Figures: 3
Number of Tables: 0

ISSN: 0030-3755 (Print)
eISSN: 1423-0267 (Online)

For additional information: http://www.karger.com/OPH


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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