Adenovirus-Mediated Expression of Brain-Derived Neurotrophic Factor Protects Spiral Ganglion Neurons from Ototoxic DamageNakaizumi T. · Kawamoto K. · Minoda R. · Raphael Y.
KHRI, University of Michigan, Ann Arbor, Mich., USA
Hair cell loss, the most common cause of deafness, is often associated with auditory nerve degeneration. Our goal was to determine the influence of combined ciliary-derived neurotrophic factor (CNTF) and brain-derived neurotrophic factor (BDNF) gene therapy on the survival of spiral ganglion neurons (SGNs) after elimination of inner hair cells in the mature guinea pig ear. Seven days after bilateral deafening, a 5-µl suspension of CNTF and/or BDNF adenovirus vectors was injected into the left scala tympani through the round window. Animals were sacrificed 28 days after deafening, and their inner ears were prepared for SGN counts. The SGN counts revealed that BDNF alone and the combined CNTF and BDNF treatment significantly enhanced SGN survival. CNTF did not significantly enhance the protective effect of BDNF. These data present possible strategies for enhancing SGN survival in cochlear implant procedures.
University of Michigan, Department of Otolaryngology
MSRB III, Rm. 9303, 1150 W. Medical Center Dr.
Ann Arbor, MI 48109-0648 (USA)
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Received: July 23, 2003
Accepted after revision: November 7, 2003
Number of Print Pages : 9
Number of Figures : 3, Number of Tables : 0, Number of References : 84
Audiology & Neuro-Otology (Basic Research and Clinical Applications)
Vol. 9, No. 3, Year 2004 (Cover Date: May-June 2004)
Journal Editor: Manfried Hoke, Münster, Germany
ISSN: 1420–3030 (print), 1421–9700 (Online)
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