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Table of Contents
Vol. 25, No. 1-2, 2004
Issue release date: June 2004
Section title: Research Article
Tumor Biol 2004;25:31–40
(DOI:10.1159/000077721)

Immunofluorometric Assay for the Metastasis-Related Protein S100A4: Release of S100A4 from Normal Blood Cells Prohibits the Use of S100A4 as a Tumor Marker in Plasma and Serum

Flatmark K.a · Maelandsmo G.M.a · Mikalsen S.-O.b · Nustad K.c · Varaas T.c · Rasmussen H.a · Meling G.I.d · Fodstad Ø.a · Paus E.c
aDepartment of Tumor Biology and Institute for Cancer Research, bDepartment of Environmental and Occupational Cancer, cCentral Laboratory, Norwegian Radium Hospital, Oslo, dDepartment of Surgery, Akershus University Hospital, Nordbyhagen, Norway

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Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: January 08, 2003
Accepted: December 17, 2003
Published online: June 14, 2004
Issue release date: June 2004

Number of Print Pages: 10
Number of Figures: 7
Number of Tables: 0

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: http://www.karger.com/TBI

Abstract

The metastasis-related protein S100A4 is released from tumor cells, and since it is highly expressed in colorectal cancer (CRC), it could be a potential tumor marker in plasma or serum. Monoclonal antibodies (MAbs) were raised against human recombinant S100A4 and shown to detect native and recombinant antigen with high sensitivity and specificity. Using two MAbs, an immunofluorometric assay (IFMA) was established to detect S100A4 in clinical samples with high sensitivity and precision. S100A4 in plasma and serum from patients with CRC was highly influenced by sample hemolysis. Both red blood cells and mononuclear cells were found to contain S100A4, possibly contributing to the measured levels in serum and plasma. Since even very low-level hemolysis influenced the results, a potential contribution from an S100A4-expressing tumor could not be discerned, indicating that S100A4 is not suitable as a plasma or serum tumor marker for CRC. The antibodies and the IFMA may still be useful for research purposes.

© 2004 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Research Article

Received: January 08, 2003
Accepted: December 17, 2003
Published online: June 14, 2004
Issue release date: June 2004

Number of Print Pages: 10
Number of Figures: 7
Number of Tables: 0

ISSN: 1010-4283 (Print)
eISSN: 1423-0380 (Online)

For additional information: http://www.karger.com/TBI


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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