Nuclear reprogramming in mammalian somatic cell nuclear cloningTamada H. · Kikyo N.
Stem Cell Institute, Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota, Minneapolis, MN (USA)
Nuclear cloning is still a developing technique used to create genetically identical animals by somatic cell nuclear transfer into unfertilized eggs. Despite an intensive effort in a number of laboratories, the success rate of obtaining viable offspring from this technique remains less than 5%. In the past few years many investigators reported the reprogramming of specific nuclear activities in cloned animals, such as genome-wide gene expression patterns, DNA methylation, genetic imprinting, histone modifications and telomere length regulation. The results highlight the tremendous difficulty the clones face to reprogram the original differentiation status of the donor nuclei. Nevertheless, nuclei prepared from terminally differentiated lymphocytes can overcome this barrier and produce apparently normal mice. Study of this striking nuclear reprogramming activity should significantly contribute to our understanding of cell differentiation in more physiological settings.
Request reprints from: Dr. Nobuaki Kikyo, Stem Cell Institute
Division of Hematology, Oncology and Transplantation
Department of Medicine, University of Minnesota, MMC 716
420 Delaware St. SE, Minneapolis, MN 55455 (USA)
telephone: +1-612-624-0498; fax: +1-612-624-2436
This work is supported by the NIH (GM068027), the American Cancer Society (IRG-58-001-43-IRG40), the Minnesota Medical Foundation, and the Graduate School of the University of Minnesota.
Received 6 October 2003;
manuscript accepted 12 November 2003.
Number of Print Pages : 7
Number of Figures : 0, Number of Tables : 4, Number of References : 93
Cytogenetic and Genome Research
Formerly ‘Cytogenetics and Cell Genetics’
Vol. 105, No. 2-4, Year 2004 (Cover Date: 2004)
Journal Editor: H.P. Klinger, Bronx, N.Y.; M. Schmid, Würzburg
ISSN: 1424–8581 (print), 1424–859X (Online)
For additional information: http://www.karger.com/journals/cgr