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Table of Contents
Vol. 24, No. 3, 2004
Issue release date: May – June
Section title: Original Report: Laboratory Investigation
Am J Nephrol 2004;24:316–321
(DOI:10.1159/000078463)

Effect of Haluronan-Supplemented Dialysate on in vitro Function of Human Peritoneal Mesothelial Cells

Bręborowicz A.a · Pyda M.a · Moberly J.c · Martis L.c · Oreopoulos D.b
aDepartment of Pathophysiology, Poznan Medical School, Poznan, Poland; bDivision of Nephrology, Department of Medicine, University of Toronto, Toronto, Ont., Canada; cBaxter Healthcare Corporation, McGraw Park, Ill., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Report: Laboratory Investigation

Received: March 08, 2004
Accepted: March 24, 2004
Published online: July 06, 2004
Issue release date: May – June

Number of Print Pages: 6
Number of Figures: 2
Number of Tables: 2

ISSN: 0250-8095 (Print)
eISSN: 1421-9670 (Online)

For additional information: http://www.karger.com/AJN

Abstract

Background: Addition of hyaluronan (HA) to the dialysis solution has been suggested as a means to protect the peritoneum from injury during peritoneal dialysis (PD). Methods: Concentrations of inflammatory mediators were determined in dialysate samples obtained from PD patients after 6-hour dwells with glucose-based (13.6 g/l) solution containing 0.1 and 0.5 g/l of exogenous high-molecular-weight HA. We additionally evaluated the effect of HA-supplemented dialysate, drained after dwell in PD patients, on function of human peritoneal mesothelial cells (MC) in in vitro culture. Results: Concentration of nitrites was significantly higher in HA 0.5 g/l supplemented dialysate (+43%, p < 0.05) as compared to control. Levels of monocyte chemoattractant protein (MCP-1), soluble intercellular adhesive molecule (s-ICAM), vascular endothelial growth factor (VEGF) and fibronectin were comparable in all the studied groups. However, when MC were exposed in in vitro conditions for 24 h to the studied dialysates, we observed that HA containing fluids inhibited the synthesis of MCP-1, s-ICAM, VEGF and fibronectin in these cells. HA-supplemented dialysate accelerated growth rate of in vitro proliferating MC. Conclusion: High-molecular-weight HA added to the dialysis fluid exerts anti-inflammatory and antifibrotic actions on the in vitro cultured MC and accelerates their growth rate what may be important for peritoneal healing during PD.

© 2004 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Report: Laboratory Investigation

Received: March 08, 2004
Accepted: March 24, 2004
Published online: July 06, 2004
Issue release date: May – June

Number of Print Pages: 6
Number of Figures: 2
Number of Tables: 2

ISSN: 0250-8095 (Print)
eISSN: 1421-9670 (Online)

For additional information: http://www.karger.com/AJN


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