The present investigation was conducted in order to determine whether lysophosphatidic acid (LPA) induces itch-scratch responses (ISRs) in mice. Intradermal administration of LPA induces ISRs; furthermore, the time course for LPA-induced ISRs was similar to that for histamine-induced responses. Comparative study of the pruritogenic activity revealed that histamine possessed a potent effect characterized by a dose-response relationship; however, prostaglandin D2 failed to induce this response. Pretreatment with ketotifen, a histamine H1 receptor antagonist, and capsaicin inhibited LPA-induced ISRs. Additionally, LPA-induced ISRs were abolished by Y-27632, an inhibitor of Rho-associated protein kinase (ROCK). These findings suggest that LPA-induced ISRs are attributable to histamine- and substance-P-mediated pathways. Moreover, the Rho/ROCK-mediated pathway may be involved.
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Received: August 21, 2003
Accepted after revision: February 12, 2004
Number of Print Pages : 6
Number of Figures : 5, Number of Tables : 0, Number of References : 30
Pharmacology (International Journal of Experimental and Clinical Pharmacology)
Vol. 72, No. 1, Year 2004 (Cover Date: September 2004)
Journal Editor: Donnerer, J. (Graz)
ISSN: 0031–7012 (print), 1423–0313 (Online)
For additional information: http://www.karger.com/pha
Article / Publication Details
Published online: 8/6/2004
Number of Print Pages: 6
Number of Figures: 5
Number of Tables: 0
ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)
For additional information: http://www.karger.com/PHA
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