Autoimmune Responses to Amyloid Structures of Aβ(25–35) Peptide and Human Lysozyme in the Serum of Patients with Progressive Alzheimer’s DiseaseGruden M.A.a · Davudova T.B.b · Mališauskas M.f · Zamotin V.V.f · Sewell R.D.E.e · Voskresenskaya N.I.c · Kostanyan I.A.d · Sherstnev V.V.a · Morozova-Roche L.A.f
aP.K. Anokhin Institute of Normal Physiology, RAS, bInstitute of General Pathology and Pathophysiology, cState Scientific Center of Psychiatric Health RAMS, and dM.M. Shemykin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia; eWelsh School of Pharmacy, Cardiff University, Cardiff, UK; fDepartment of Medical Biochemistry and Biophysics, Umeå University, Umeå, Sweden
We have found an increased level of serum antibodies to the prefibrillar structures of both Aβ(25–35) peptide and human lysozyme in Alzheimer’s disease (AD) patients compared to age-matched controls, indicating that autoimmunity is implicated in AD. In the serum of AD patients with a long-term duration (>15 years) the titer of serum antibodies to aggregates of Aβ(25–35) peptide increased by approximately 5-fold, whilst the antibody titer to lysozyme protofilaments decreased by approximately 8-fold compared to patients with AD duration of <5 years. The content of immunoglobulins of the A, G and M types declined, particularly in AD duration of >15 years. An increase in the concentration of immune complexes and higher lysozyme activity was detected in the serum of all patients and this was suggestive of an inflammatory reaction. We propose that the autoimmune response to different amyloid structures in AD can be viewed as a clearance pathway targeting amyloid development. Autoimmune response can be exploited as a marker of ongoing protein aggregation and hence be used as a diagnostic feature of AD.
© 2004 S. Karger AG, Basel
Department of Medical Biochemistry and Biophysics, Umeå University
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Accepted: January 30, 2004
Published online: June 21, 2004
Number of Print Pages : 7
Number of Figures : 1, Number of Tables : 2, Number of References : 36
Dementia and Geriatric Cognitive Disorders
Vol. 18, No. 2, Year 2004 (Cover Date: Released August 2004)
Journal Editor: V. Chan-Palay, New York, N.Y.
ISSN: 1420–8008 (print), 1421–9824 (Online)
For additional information: http://www.karger.com/journals/dem