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Table of Contents
Vol. 71, No. 4, 2004
Issue release date: July – August
Section title: Basic Science Investigations
Respiration 2004;71:402–409
(DOI:10.1159/000079647)

Exposure of Differentiated Airway Epithelial Cells to Volatile Smoke in vitro

Beisswenger C. · Platz J. · Seifart C. · Vogelmeier C. · Bals R.
Department of Internal Medicine, Division of Pulmonary Diseases, Philipps University of Marburg, Marburg, Germany

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Article / Publication Details

First-Page Preview
Abstract of Basic Science Investigations

Received: September 09, 2003
Accepted: February 19, 2004
Published online: August 13, 2004
Issue release date: July – August

Number of Print Pages: 8
Number of Figures: 6
Number of Tables: 0

ISSN: 0025-7931 (Print)
eISSN: 1423-0356 (Online)

For additional information: http://www.karger.com/RES

Abstract

Background: Cigarette smoke (CS) is the predominant pathogenetic factor in the development of chronic bronchitis and chronic obstructive pulmonary disease. The knowledge about the cellular and molecular mechanisms underlying the smoke-induced inflammation in epithelial cells is limited. Objectives: The aim of this study was to develop an in vitro model to monitor the effects of volatile CS on differentiated airway epithelial cells. Methods: The airway epithelial cell line MM-39 and primary human bronchial epithelial cells were cultivated as air-liquid interface cultures and exposed directly to volatile CS. We used two types of exposure models, one using ambient air, the other using humidified and warm air. Cytokine levels were measured by quantitative PCR and ELISA. Phosphorylation of p38 MAP kinase was assessed by Western blot analysis. To reduce the smoke-induced inflammation, antisense oligonucleotides directed against the p65 subunit of NF-ĸB were applied. Results: Exposure of epithelia to cold and dry air resulted in a significant inflammatory response. In contrast, exposure to humidified warm air did not elicit a cellular response. Stimulation with CS resulted in upregulation of mRNA for IL-6 and IL-8 and protein release. Exposure to CS combined with heat-inactivated bacteria synergistically increased levels of the cytokines. Reactions of differentiated epithelial cells to smoke are mediated by the MAP kinase p38 and the transcription factor NF-ĸB. Conclusions: We developed an exposure model to examine the consequences of direct exposure of differentiated airway epithelial cells to volatile CS. The model enables to measure the cellular reactions to smoke exposure and to determine the outcome of therapeutic interventions.

© 2004 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Basic Science Investigations

Received: September 09, 2003
Accepted: February 19, 2004
Published online: August 13, 2004
Issue release date: July – August

Number of Print Pages: 8
Number of Figures: 6
Number of Tables: 0

ISSN: 0025-7931 (Print)
eISSN: 1423-0356 (Online)

For additional information: http://www.karger.com/RES


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