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Table of Contents
Vol. 33, No. 4, 2003
Issue release date: July – August
Section title: Original Paper
Pathophysiol Haemost Thromb 2003;33:202–205

Ecarin Chromogenic Assay – A New Method for Quantitative Determination of Direct Thrombin Inhibitors Like Hirudin

Lange U.a · Nowak G.b · Bucha E.a
aHaemoSys GmbH and bResearch Unit ‘Pharmacological Hemostaseology’, Medical Faculty, Friedrich Schiller University, Jena, Germany
email Corresponding Author

Dr. Ute Lange

HaemoSys GmbH

Winzerlaer Strasse 2

DE–07745 Jena (Germany)

Tel. +49 36 41 50 83 21, Fax +49 36 41 50 83 01, E-Mail u.lange@haemosys.de

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A new sensitive and precise method for quantitative determination of direct thrombin inhibitors is described, the ecarin chromogenic assay (ECA). Ecarin is used as the specific prothrombin-activating principle. The cleavage of a chromogenic substrate by meizothrombin is inhibited in a concentration-dependent fashion by direct thrombin inhibitors. For the ECA, the linear measuring range is about 0.1–3.0 µg hirudin/ml plasma. Coefficients of variations between 2.3 and 4% over the whole concentration range were achieved. The ECA has proved to be more sensitive than the compared tests (ecarin clotting time and a thrombin-based chromogenic assay); a detection limit of 0.011 µg hirudin/ml and a quantitation limit of 0.032 µg hirudin/ml were calculated. The ECA is independent of the variations of the coagulation variables fibrinogen and prothrombin. Neither heparin nor oral anticoagulants interfere with the ECA.

© 2004 S. Karger AG, Basel

Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: July 26, 2004
Accepted: September 14, 2004
Published online: December 02, 2004
Issue release date: July – August

Number of Print Pages: 8
Number of Figures: 7
Number of Tables: 2

ISSN: 1424-8832 (Print)
eISSN: 1424-8840 (Online)

For additional information: http://www.karger.com/PHT

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