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Vol. 178, No. 2, 2004
Issue release date: 2004
Section title: Original Paper
Cells Tissues Organs 2004;178:87–92
(DOI:10.1159/000081719)

Comparison of Viable Cell Yield from Excised versus Aspirated Adipose Tissue

von Heimburg D.a · Hemmrich K.a · Haydarlioglu S.a · Staiger H.b · Pallua N.a
aDepartment of Plastic Surgery and Hand Surgery, Burn Center, University Hospital, Aachen University of Technology, Aachen, and bDepartment of Internal Medicine IV, Eberhard Karls University Tübingen, Tübingen, Germany

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 8/3/2004
Published online: 12/13/2004
Issue release date: 2004

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

The correction of soft-tissue defects by adipose tissue transplantation often produces poor and unpredictable results. The implantation of isolated and cultured preadipocytes offers a solution to this problem since these cells differentiate into adipocytes when implanted in vivo. A field of major interest is to maximize the yield of preadipocytes isolated from adipose tissue showing only low contamination with other cell types. Aspiration and excision are two concurrent clinical ways of harvesting adipose tissue for the isolation of preadipocytes. This tissue is usually discarded after surgery. In this study, the yield of preadipocytes obtained from liposuction material was compared to that of excised adipose tissue. Furthermore, we determined the loss of precursor cells if isolation of preadipocytes was delayed for 24 h. Preadipocytes were isolated from the stromal cell fraction of human subcutaneous adipose tissue samples. Harvesting of adipose tissue by suction was performed according to the Coleman procedure (manually applied negative pressure using a 10-ml syringe with a blunt tip cannula). Isolation was either carried out within 60 min after extraction or after storage for 24 h in culture medium at 4°C. Isolated preadipocytes were cultured for 24 h, trypsinized and counted in a Neubauer chamber. Our results show clearly that the yield of preadipocytes isolated from liposuction material (within 60 min after extraction and after 24 h of storage) is higher than the cell yield from excised adipose tissue. Overnight storage for 24 h leads to a significant loss of preadipocytes in excised tissue but not in liposuction material. The high yield of cells isolated from liposuction material proves that extraction by suction does not damage the stromal cell fraction in the adipose tissue. If cell isolation is not performed immediately after the operation, liposuction material is clearly the better alternative for storage.


  

Author Contacts

Dennis von Heimburg, MD, PhD
Praxisklinik Kaiserplatz, Kaiserstrasse 14
DE–60311 Frankfurt am Main (Germany)
Tel. +49 69 92884747, Fax +49 69 92884744
E-Mail dvheimburg@praxisklinik-kaiserplatz.de

  

Article Information

D.v.H. and K.H. are both first authors.

Accepted after revision: August 1, 2004
Number of Print Pages : 6
Number of Figures : 1, Number of Tables : 1, Number of References : 33

  

Publication Details

Cells Tissues Organs (in vivo, in vitro)

Vol. 178, No. 2, Year 2004 (Cover Date: 2004)

Journal Editor: H.-W. Denker, Essen; A.W. English, Atlanta, Ga.
ISSN: 1422–6405 (print), 1422–6421 (Online)

For additional information: http://www.karger.com/cto


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 8/3/2004
Published online: 12/13/2004
Issue release date: 2004

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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