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Table of Contents
Vol. 101, No. 2, 2005
Issue release date: October 2005
Section title: Original Paper
Nephron Exp Nephrol 2005;101:e67–e74
(DOI:10.1159/000086500)

Oxidative Stress Decreases Klotho Expression in a Mouse Kidney Cell Line

Mitobe M.a · Yoshida T.a, b · Sugiura H.a · Shirota S.a · Tsuchiya K.a · Nihei H.a
Departments of aMedicine IV, and bBlood Purification, Tokyo Women’s Medical University, Tokyo, Japan

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: February 28, 2005
Accepted: April 04, 2005
Published online: June 22, 2005
Issue release date: October 2005

Number of Print Pages: 1
Number of Figures: 6
Number of Tables: 0

ISSN: (Print)
eISSN: 1660-2129 (Online)

For additional information: http://www.karger.com/NEE

Abstract

Background/Aims: Defects in klotho gene expression in the mouse result in a syndrome that resembles human aging. We recently identified expression of klotho in a mouse inner medullary collecting duct (mIMCD3) cell line for the first time, and in the present study we explored the physiological relevance of the regulation of klotho expression in the presence of oxidant stress injury. Methods: Klotho expression was analyzed by real-time PCR, Western blot, and immuocytochemical staining during exposure to hydrogen peroxide (H2O2). Overexpression of the klotho gene was induced by klotho adenoviruses, and the number of apoptotic cells was counted by flowcytometry. Results: Oxidant stress injury by H2O2 dose-dependently reduced klotho expression and diminished klotho staining. There were fewer apoptotic cells among the klotho-transfected cells than among the control cells. Conclusion: Klotho is expressed in cell line mIMCD3, and the klotho gene may be involved in the process of oxidative stress injury and apoptosis in this cell line.

© 2005 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: February 28, 2005
Accepted: April 04, 2005
Published online: June 22, 2005
Issue release date: October 2005

Number of Print Pages: 1
Number of Figures: 6
Number of Tables: 0

ISSN: (Print)
eISSN: 1660-2129 (Online)

For additional information: http://www.karger.com/NEE


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