Influence of Cranberry Juice on Glucan-Mediated Processes Involved in Streptococcus mutans Biofilm DevelopmentKoo H. · Nino de Guzman P. · Schobel B.D. · Vacca Smith A.V. · Bowen W.H.
Center for Oral Biology and Eastman Department of Dentistry, University of Rochester Medical Center, Rochester, N.Y., USA
Cranberry juice (CJ) has biological properties that may provide health benefits. In this study, we investigated the influence of CJ (pH 5.5) on several activities in vitro associated with the development of Streptococcus mutans UA159 biofilms. The ability of CJ to influence the adherence of S. mutans to either saliva- (sHA) or glucan-coated hydroxyapatite (gsHA), and to inhibit the glucan production by purified glucosyltransferases adsorbed to sHA was determined. For the adherence assays, we used both uncoated and saliva-coated bacterial cells. Furthermore, we examined whether CJ interferes with the viability, development, polysaccharide composition and acidogenicity of S. mutans biofilms. A solution containing equivalent amounts of glucose, fructose and organic acids at pH 5.5 was used as negative control. The adherence of S. mutans (uncoated and saliva-coated) to either sHA or gsHA treated with 25% CJ (v/v) was remarkably reduced (40–85% inhibition compared to control: p < 0.05), indicating that CJ effectively blocked the bacterial adherence to binding sites in salivary pellicle and in glucans. In contrast, when the bacterial cells alone were treated with CJ they adhered to the similar untreated surfaces. Cranberry juice (25%, v/v) also inhibited the activities of surface-adsorbed GTF B and C (70–80% inhibition compared to control, p < 0.05). The effect of CJ on the viability of microorganisms in biofilms was not significant. Biofilm formation and accumulation were significantly reduced by topical applications of 25% CJ (v/v) twice daily with 1-min exposures (p < 0.05). The biomass and insoluble glucan content of the biofilms in addition to its acidogenicity were significantly reduced by cranberry treatments (p < 0.05). Our data show that cranberry juice inhibited glucan-mediated biofilm development and acid production, and holds promise as a natural product to prevent biofilm-related oral diseases.
© 2006 S. Karger AG, Basel
University of Rochester Medical Center
Center for Oral Biology and Eastman Department of Dentistry
601 Elmwood Avenue, Box 611, Rochester, NY 14642 (USA)
Tel. +1 585 273 4216, Fax +1 585 276 0190, E-Mail Hyun_Koo@urmc.rochester.edu
Received: October 8, 2004
Accepted after revision: February 18, 2005
Number of Print Pages : 8
Number of Figures : 2, Number of Tables : 2, Number of References : 29
Vol. 40, No. 1, Year 2006 (Cover Date: December 2005)
Journal Editor: Shellis, R.P.(Bristol)
ISSN: 0008–6568 (print), 1421–976X (Online)
For additional information: http://www.karger.com/CRE