Effect of Berberine on Interleukin 8 and Monocyte Chemotactic Protein 1 Expression in a Human Retinal Pigment Epithelial Cell LineCui H.-S. · Hayasaka S. · Zhang X.-Y. · Hayasaka Y. · Chi Z.-L. · Zheng L.-S.
Departments of aOphthalmology and bPathology, Toyama Medical and Pharmaceutical University, Toyama, Japan
Purpose: The aims of this study were to examine the effects of berberine, an alkaloid isolated from some medicinal herbs, on interleukin 8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) expression in a human retinal pigment epithelial cell line (ARPE-19) stimulated with interleukin 1β (IL-1β) or tumor necrosis factor α (TNF-α). Methods: ARPE-19 cells were cultured to confluence. Berberine and IL-1β or TNF-α were added to the medium. IL-8 mRNA and MCP-1 mRNA were measured by semiquantitative reverse-transcription polymerase chain reaction and real-time polymerase chain reaction. IL-8 and MCP-1 protein concentrations in the media were measured using enzyme-linked immunosorbent assay. Results: Berberine dose-dependently inhibited IL-8 mRNA and MCP-1 mRNA expression of the cells and protein levels in the media stimulated with IL-1β or TNF-α. Conclusion: These findings indicate that berberine dose-dependently inhibited the expression of IL-8 and MCP-1 induced by IL-1β or TNF-α.
Department of Ophthalmology, Toyama Medical and Pharmaceutical University
Toyama 930-0194 (Japan)
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Received: August 10, 2005
Accepted after revision: November 21, 2005
Published online: January 2, 2006
Number of Print Pages : 9
Number of Figures : 8, Number of Tables : 0, Number of References : 29
Ophthalmic Research (Journal for Research in Experimental and Clinical Ophthalmology)
Vol. 38, No. 3, Year 2006 (Cover Date: May 2006)
Journal Editor: Pleyer, U. (Berlin)
ISSN: 0030–3747 (print), 1423–0259 (Online)
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