Glucose Permeability of Human, Bovine, and Porcine Corneas in vitroMyung D. · Derr K. · Huie P. · Noolandi J. · Ta K.P. · Ta C.N.
Department of Ophthalmology, Stanford University, Stanford, Calif., USA
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Purpose: To measure glucose flux across human, bovine, and porcine corneas and to determine the diffusion coefficient of each type of cornea. Methods: Diffusion of glucose across human (n = 8), bovine (n = 7), and pig corneas (n = 8) was measured using a modified blind well chamber apparatus (Boyden chamber). Dialysis membranes (n = 7) and nonporous Mylar membranes (n = 7) were used as positive and negative controls, respectively. Glucose concentrations were measured at 30-min intervals with a commercially available glucose meter. Results: The diffusion of glucose through corneas in vitro was calculated by a simple Fickian diffusion model. The diffusion coefficient of glucose is highest for the human cornea (DHC = 3.0 ± 0.2 × 10–6 cm2/s) followed by porcine corneas (DPC = 1.8 ± 0.6 × 10–6 cm2/s) and bovine corneas (DBC = 1.6 ± 0.1 × 10–6 cm2/s) (p < 0.05). The diffusion coefficients of all tested corneas were significantly higher (p < 0.05) than that of dialysis membrane (DDM = 3.4 ± 0.2 × 10–7 cm2/s). Conclusion: The glucose diffusion coefficients of human, bovine, and porcine corneas are on the order of 10–6. Human corneas have higher permeability to glucose than either porcine or bovine corneas.
© 2006 S. Karger AG, Basel
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