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Imprinted Genes/Domains

Aberrant regulation of imprinted gene expression in Gtl2lacZ mice

Sekita Y.a, b · Wagatsuma H.b · Irie M.a · Kobayashi S.b · Kohda T.a · Matsuda J.c · Yokoyama M.d · Ogura A.e · Schuster-Gossler K.f · Gossler A.f · Ishino F.a · Kaneko-Ishino T.g

Author affiliations

aDepartment of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo (Japan); bDepartment of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama (Japan); cDepartment of Veterinary Sciences, National Institute of Infectious Diseases, Tokyo (Japan); dMitsubishi Kagaku Institute of Life Sciences, Machida (Japan); eRIKEN BioResource Center, Tsukuba (Japan); fInstitute for Molecular Biology OE5250, Medizinische Hochschule Hannover, Hannover (Germany); gSchool of Health Sciences, Tokai University, Isehara (Japan)

Related Articles for ""

Cytogenet Genome Res 113:223–229 (2006)

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Article / Publication Details

First-Page Preview
Abstract of Imprinted Genes/Domains

Published online: March 30, 2006
Issue release date: March 2006

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

The imprinted region on mouse distal chromosome 12 covers about 1 Mb and contains at least three paternally expressed genes (Pegs: Peg9/Dlk1, Peg11/Rtl1, and Dio3) and four maternally expressed genes (Megs: Meg3/Gtl2, antiPeg11/antiRlt1, Meg8/Rian, and Meg9/Mirg). Gtl2lacZ (Gene trap locus 2) mice have a transgene (TG) insertion 2.3 kb upstream from the Meg3/Gtl2 promoter and show about 40% growth retardation when the TG-inserted allele is paternally derived. Quantitative RT-PCR experiments showed that the expression levels of Pegs in this region were reduced below 50%. These results are consistent with the observed phenotype in Gtl2lacZ mice, because at least two Pegs(Peg9/Dlk1 and Dio3) have growth-promoting effects. The aberrant induction of Megs from silent paternal alleles was also observed in association with changes in the DNA methylation level of a differentially methylated region (DMR) located around Meg3/Gtl2 exon 1. Interestingly, a 60∼80% reduction in all Megs was observed when the TG was maternally derived, although the pups showed no apparent growth or morphological abnormalities. Therefore, the paternal or maternal inheritance of the TG results in the down-regulation in cis of either Pegs or Megs, respectively, suggesting that the TG insertion influences the mechanism regulating the entire imprinted region.

© 2006 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Imprinted Genes/Domains

Published online: March 30, 2006
Issue release date: March 2006

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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