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Vol. 12, No. 6, 2005
Issue release date: March 2006
Section title: Original Paper
Neuroimmunomodulation 2005;12:321–338
(DOI:10.1159/000091126)

Nuclear Factor-ĸB- and Glucocorticoid Receptor α- Mediated Mechanisms in the Regulation of Systemic and Pulmonary Inflammation during Sepsis and Acute Respiratory Distress Syndrome

Evidence for Inflammation-Induced Target Tissue Resistance to Glucocorticoids

Meduri G.U.a · Muthiah M.P.a · Carratù P.a · Eltorky M.a · Chrousos G.P.b
aMemphis Lung Research Program, Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, University of Tennessee Health Science Center, Memphis, Tenn., USA, and bFirst Department of Pediatrics, Athens University Medical School, Athens, Greece

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 9/12/2005
Accepted: 11/6/2005
Published online: 1/17/2012
Issue release date: March 2006

Number of Print Pages: 18
Number of Figures: 9
Number of Tables: 1

ISSN: 1021-7401 (Print)
eISSN: 1423-0216 (Online)

For additional information: http://www.karger.com/NIM

Abstract

To test the hypothesis that the interaction between nuclear factor-ĸB (NF-ĸB) and glucocorticoid receptor α (GRα) is a key pathogenetic mechanism regulating the progression of systemic and pulmonary inflammation in sepsis and acute respiratory distress syndrome (ARDS), we used an ex vivo model of systemic inflammation. Naïve peripheral blood leukocytes (PBL) were exposed to longitudinal (days 1–10) plasma samples from ARDS patients divided into three groups based on physiological improvement and clinical outcome by days 7–10: improvers, nonimprovers-survivors, and nonimprovers-nonsurvivors. In a separate group of nonimprovers-survivors, we correlated the severity of lung histopathology with the intensity of NF-ĸB and GRα nuclear staining in immunohistochemistry analysis of lung tissue obtained by open lung biopsy. We found that exposure of naïve cells to longitudinal plasma samples led to divergent directions in NF-ĸB and GRα activation that reflected the severity of systemic inflammation. Plasma samples from improvers with declining cytokine levels over time elicited a progressive increase in all measured aspects of glucocorticoid (GC)-induced GRα-mediated activity (p = 0.0001) and a correspondent reduction in NF-ĸB nuclear binding (p = 0.0001) and transcription of TNF-α and IL-1β (regulated, GRα-driven response). In contrast, plasma samples from nonimprovers with sustained elevation in cytokine levels over time elicited only a modest longitudinal increase in GC-GRα-mediated activity (p = 0.04) and a progressive increase in NF-ĸB nuclear binding over time (p = 0.0001) that was most striking in nonsurvivors (dysregulated, NF-ĸB-driven response). By days 3–5, no overlap was observed between groups for NF-ĸB and GC-GRα nuclear binding. In immunohistochemistry analyses, lung tissues of patients with severe versus mild ARDS had a higher intensity of NF-ĸB nuclear staining (13 ± 1.3 vs. 7 ± 2.9; p = 0.01) and a lower ratio of GRα:NF-ĸB in nuclear staining (0.5 ± 0.2 vs. 1.5 ± 0.2; p = 0.007). In conclusion, we demonstrated that the ability of GC-GRα to downregulate NF-ĸB activation is critical for the resolution of systemic and pulmonary inflammation in ARDS. The findings provide a rationale for the use of prolonged GC treatment in early ARDS.

© 2005 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 9/12/2005
Accepted: 11/6/2005
Published online: 1/17/2012
Issue release date: March 2006

Number of Print Pages: 18
Number of Figures: 9
Number of Tables: 1

ISSN: 1021-7401 (Print)
eISSN: 1423-0216 (Online)

For additional information: http://www.karger.com/NIM


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