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Vol. 182, No. 1, 2006
Issue release date: April 2006
Section title: Original Paper
Cells Tissues Organs 2006;182:1–11
(DOI:10.1159/000091713)

Characterization of Osteogenically Induced Adipose Tissue-Derived Precursor Cells in 2-Dimensional and 3-Dimensional Environments

Leong D.T. · Khor W.M. · Chew F.T. · Lim T.-C. · Hutmacher D.W.
aDepartment of Biological Sciences, bDepartment of Mechanical Engineering, cDivision of Bioengineering and dDepartment of Orthopedic Surgery, National University of Singapore and eDivision of Plastic Surgery, National University Hospital, Singapore, Singapore

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 12/16/2005
Published online: 4/20/2006

Number of Print Pages: 11
Number of Figures: 5
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO

Abstract

Earlier reports on a putative precursor cell population in adipose tissue showed differentiation along several mesodermal lineages, leading some to think that adipose tissue can be a source of cells applicable in regenerative medicine. However, characterizations of these adipose-derived precursor cells (ADPC) in the 3-dimensional (3-D) environment, especially within the area of bone-specific composite scaffolds, have been lacking. In this study, ADPC plated on culture flasks or seeded on medical grade polycaprolactone-tricalcium phosphate scaffolds (mPCL-CaP) were able to differentiate along the osteogenic lineages in both 2-D and 3-D environments as assessed with immunohistochemistry of osteo-related proteins, reverse transcriptase-polymerase chain reactions and alkaline phosphatase assay. The mPCL-CaP scaffolds provided adipose-derived cells (ADC) with a suitable environment as determined by DNA and metabolic assays, light, confocal and scanning electron microscopy. Flow cytometry revealed ADC to be CD29+, CD44+, CD73+, CD90+ and CD14–, CD31–, CD34–, CD45–, CD71–, and therefore showed the absence of hematopoietic stem cells but possibly the presence of pericytes and mescenchymal stem cells with osteogenic potential. The results of this study demonstrated the potential of using ADPC in combination with mPCL-CaP scaffolds for bone regenerative medicine.


  

Author Contacts

Dietmar Werner Hutmacher
Division of Bioengineering, National University of Singapore
9 Engineering Drive 1, EA 03-12
Singapore 117576 (Singapore)
Tel. +65 6874 1036, Fax +65 6872 3069, E-Mail biedwh@nus.edu.sg

  

Article Information

Work was carried out at the Tissue Engineering Laboratory, Division of Bioengineering, National University of Singapore.

Accepted after revision: December 16, 2005
Number of Print Pages : 11
Number of Figures : 5, Number of Tables : 1, Number of References : 50

  

Publication Details

Cells Tissues Organs (in vivo, in vitro)

Vol. 182, No. 1, Year 2006 (Cover Date: April 2006)

Journal Editor: Denker, H.-W. (Essen)
ISSN: 1422–6405 (print), 1422–6421 (Online)

For additional information: http://www.karger.com/CTO


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 12/16/2005
Published online: 4/20/2006

Number of Print Pages: 11
Number of Figures: 5
Number of Tables: 1

ISSN: 1422-6405 (Print)
eISSN: 1422-6421 (Online)

For additional information: http://www.karger.com/CTO


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