Rhinoviral infections belong to the most frequent
human infections characterized by common cold,
chronic bronchitis, exacerbations of asthma, otitis
media and sinusitis. Here, we define molecular
mechanisms that mediate infections of human
epithelial cells with human rhinovirus strain 14 (RV14).
We demonstrate that RV14 activates p38-MAPKinase
(p38-K) in a biphasic time course. Early
stimulation of p38-K by RV14 was observed a few
minutes after initiation of the infection, while the late
increase of p38-K activity occurred 7-12 hrs upon
infection. The stimulation of p38-K was mediated by
the small G-protein RhoA,which was activated by
RV14. Transfection of a genetic construct preventing
RhoA activation blocked RV14-induced p38-K
activation. Further, integrity of cholesterol and
sphingolipid-enriched membrane domains was
required for RV14-mediated p38-K activation, which
was inhibited by destruction of membrane rafts. The
data indicate that RV employs a signaling cascade
from membrane rafts via the small G-protein RhoA to
p38-K to infect human cells.
Article / Publication Details
Published online: March 14, 2006
Issue release date: March 2006
Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0
ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)
For additional information: http://www.karger.com/CPB
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