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Vol. 140, No. 2, 2006
Issue release date: May 2006
Section title: Original Paper
Int Arch Allergy Immunol 2006;140:139–149
(DOI:10.1159/000092533)

Enhanced Frequency of CD18- and CD49b-Expressing T Cells in Peripheral Blood of Asthmatic Patients Correlates with Disease Severity

Rahmoun M. · Foussat A. · Groux H. · Pène J. · Yssel H. · Chanez P.
aINSERM U454, and bClinique des Maladies Respiratoires CHU de Montpellier, Montpellier, and cTxCell, Sophia Antipolis, France

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 9/15/2005
Accepted: 1/10/2006
Published online: 5/11/2006

Number of Print Pages: 11
Number of Figures: 7
Number of Tables: 1

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA

Abstract

Background: Results from a transcriptome analysis of human CD4+ T regulatory type 1 (Tr1) clones have indicated that transcripts for the integrins CD18 and CD49b are overexpressed in these cells. The aim of this study was to investigate whether the presence of T cells concomitantly expressing these molecules could be detected in asthmatic patients and represent Tr1 cells. Methods:Expression of CD18 and CD49b was analyzed by flow cytometry on peripheral blood mononuclear cells from asthmatic patients of various severity and healthy subjects. The cytokine production profile of purified CD4+ CD18high CD49b+ T cells was analyzed by ELISA. The effect of glucocorticoid treatment on the expression of CD18 and CD49b was determined. Results: The frequency of peripheral blood CD18high CD49b+ T cells was significantly elevated in severe asthmatic patients, as compared with both mild asthmatic and healthy donors, and was diminished in asthmatic patients with a controlled status of the disease. Neither short-course oral glucocorticoid treatment of asthmatic patients ex vivo, nor culture of their peripheral blood mononuclear cells with dexamethasone in vitro, increased the frequency of CD18high CD49b+ T cells, indicating that their presence seems to be independent from recent anti-inflammatory treatment. However, purified CD4+ CD18high CD49b+ T cells from these patients, in contrast to those from healthy donors, lacked the production of the immunosuppressive cytokine interleukin-10. Conclusion: In contrast to healthy donors, peripheral blood CD18high CD49b+ T cells of asthmatic patients do not fulfill the phenotypic criteria of Tr1 cells. Nevertheless, the presence of elevated numbers of peripheral blood CD18high CD49b+ T cells is characteristic for patients with severe and uncontrolled asthma.


  

Author Contacts

Correspondence to: Dr. Hans Yssel
INSERM U454, CHU Arnaud de Villeneuve
371, Avenue Doyen Gaston Giraud
FR–34295 Montpellier, Cedex 5 (France)
Tel. +33 4 67 41 52 12, Fax +33 4 67 63 28 55, E-Mail yssel@montp.inserm.fr

  

Article Information

Received: September 15, 2005
Accepted after revision: January 10, 2006
Published online: April 4, 2006
Number of Print Pages : 11
Number of Figures : 7, Number of Tables : 1, Number of References : 29

  

Publication Details

International Archives of Allergy and Immunology

Vol. 140, No. 2, Year 2006 (Cover Date: May 2006)

Journal Editor: Valenta, R. (Vienna)
ISSN: 1018–2438 (print), 1423–0097 (Online)

For additional information: http://www.karger.com/IAA


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 9/15/2005
Accepted: 1/10/2006
Published online: 5/11/2006

Number of Print Pages: 11
Number of Figures: 7
Number of Tables: 1

ISSN: 1018-2438 (Print)
eISSN: 1423-0097 (Online)

For additional information: http://www.karger.com/IAA


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