Journal Mobile Options
Table of Contents
Vol. 43, No. 4, 2006
Issue release date: July 2006
Section title: Research Paper
J Vasc Res 2006;43:338–346
(DOI:10.1159/000093606)

Different Vascular Smooth Muscle Cell Apoptosis in the Human Internal Mammary Artery and the Saphenous Vein

Implications for Bypass Graft Disease

Frischknecht K. · Greutert H. · Weisshaupt C. · Kaspar M. · Yang Z. · Lüscher T.F. · Carrel T.P. · Tanner F.C.
aCardiovascular Research, Physiology Institute, and bCenter for Integrative Human Physiology, University of Zürich, cCardiology, Cardiovascular Center, University Hospital of Zürich, Zürich, dDepartment of Medicine, Division of Physiology, University Fribourg, Fribourg, and eClinic for Cardiovascular Surgery, University Hospital of Bern, Bern, Switzerland

Do you have an account?

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger (new!)
  • Unrestricted printing, no saving restrictions for personal use
  • Reduced rates with a PPV account
read more

Direct: USD 38.00
Account: USD 26.50

Select

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Automatic perpetual access to all articles of the subscribed year(s)
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select


Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 7/14/2005
Accepted: 3/13/2006
Published online: 7/28/2006

Number of Print Pages: 9
Number of Figures: 4
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR

Abstract

Background: The remarkable patency of internal mammary artery (MA) grafts compared to saphenous vein (SV) grafts has been related to different biological properties of the two blood vessels. We examined whether proliferation and apoptosis of vascular smooth muscle cells (VSMC) from human coronary artery bypass vessels differ according to patency rates. Methods and Results: Proliferation rates to serum or platelet-derived growth factor (PDGF)-BB were lower in VSMC from MA than SV. Surface expression of PDGF β-receptor was slightly lower, while that of α-receptor was slightly higher in MA than SV. Cell cycle distribution, expression of cyclin E, cdk2, p21, p27, p57, and cdk2 kinase activity were identical in PDGF-BB-stimulated cells from MA and SV. However, apoptosis rates were higher in MA than SV determined by lactate dehydrogenase release, DNA fragmentation, and Hoechst 33258 staining. Moreover, caspase inhibitors (Z-VAD-fmk, Boc-D-fmk) abrogated the different proliferation rates of VSMC from MA versus SV. Western blotting and GSK3-β kinase assay revealed lower Akt activity in VSMC from MA versus SV, while total Akt expression was identical. Adenoviral transduction of a constitutively active Akt mutant abrogated the different proliferation rates of VSMC from MA versus SV. Conclusions: Higher apoptosis rates due to lower Akt activity rather than different cell cycle regulation account for the lower proliferation of VSMC from MA as compared to SV. VSMC apoptosis may protect MA from bypass graft disease.


  

Author Contacts

Dr. Felix C. Tanner
Cardiovascular Research, Physiology Institute, University of Zürich
Winterthurerstrasse 190
CH–8057 Zürich (Switzerland)
Tel. +41 44 635 64 69, Fax +41 44 635 68 27, E-Mail felix.tanner@access.unizh.ch

  

Article Information

The study was supported by the Swiss National Science Foundation (grant No. 3200B0-102232/1 to F.C.T. and No. 32-51069.97/2 to T.F.L.), the Swiss Heart Foundation, the Novartis Foundation and the ­Schweizerische Rentenanstalt.

Received: July 14, 2005
Accepted after revision: March 13, 2006
Published online: May 29, 2006
Number of Print Pages : 9
Number of Figures : 4, Number of Tables : 1, Number of References : 21

  

Publication Details

Journal of Vascular Research (Incorporating 'International Journal of Microcirculation')

Vol. 43, No. 4, Year 2006 (Cover Date: July 2006)

Journal Editor: Pohl, U. (Munich)
ISSN: 1018–1172 (print), 1423–0135 (Online)

For additional information: http://www.karger.com/JVR


Article / Publication Details

First-Page Preview
Abstract of Research Paper

Received: 7/14/2005
Accepted: 3/13/2006
Published online: 7/28/2006

Number of Print Pages: 9
Number of Figures: 4
Number of Tables: 1

ISSN: 1018-1172 (Print)
eISSN: 1423-0135 (Online)

For additional information: http://www.karger.com/JVR


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.