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Table of Contents
Vol. 21, No. 5, 2006
Issue release date: August 2006
Section title: Paper
Fetal Diagn Ther 2006;21:404–409
(DOI:10.1159/000093880)

Non-Invasive Detection of Fetal Rhesus D Status: A Comparison between Polymerase Chain Reaction and Flow Cytometry

Di Simone N. · Lai M. · Rumi C. · Riccardi P. · D’Asta M. · Leone G. · Mancuso S. · Caruso A.
Departments of aObstetrics and Gynecology and bHematology, Catholic University of the Sacred Heart, Rome, Italy

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Article / Publication Details

First-Page Preview
Abstract of Paper

Received: 11/17/2004
Accepted: 9/5/2005
Published online: 8/17/2006

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 2

ISSN: 1015-3837 (Print)
eISSN: 1421-9964 (Online)

For additional information: http://www.karger.com/FDT

Abstract

Objective: A non-invasive prenatal determination of the fetal RhD status might be useful for the management of pregnancies in RhD-negative women whose partners are RhD positive. Methods: Maternal peripheral blood of 32 RhD-negative women (17–24 weeks of gestation) was collected, and circulating fetal cells were enriched by CD71 mini-magnetic activated cell sorting. The RhD status of the fetuses was assessed using multiparametric flow cytometry, and results were compared to those of reverse transcriptase (RT)-polymerase chain reaction (PCR), or PCR, which acted as control. Flow-cytometric study of fetal cells employed monoclonal antibodies directed against CD71, glycophorin A (GPA) and RhD antigens. Results: The median percentage of CD71- and RhD-positive cells was 0.83% (range 0.14–6.44%), and that of CD71 and GPA-positive cells was 10.07% (range 0.52–45.84%). Flow-cytometric analysis correlated with RT-PCR results of RNA obtained from whole maternal blood. In 1 case, an incorrect result was due to the failure of the amplification of the specific RhD band on RNA extracted from the CD71-positive fraction. In two instances, we observed false-positive results for RhD in PCR of DNA obtained from maternal plasma. Conclusion: Based on our results, flow-cytometric analysis might be proposed as a clinical tool for the non-invasive prenatal determination of the fetal RhD status independently of fetal gender.


  

Author Contacts

Alessandro Caruso, MD
Department of Obstetrics and Gynecology, Catholic University of the Sacred Heart
Largo Gemelli 8
IT–00168 Rome (Italy)
Tel. +39 06 3015 6583, Fax +39 06 305 1160, E-Mail acaruso@rm.unicatt.it

  

Article Information

Supported by a grant from the Catholic University of the Sacred Heart (D3.2, year 2001).

Received: November 17, 2004
Accepted after revision: September 5, 2005
Number of Print Pages : 6
Number of Figures : 1, Number of Tables : 2, Number of References : 27

  

Publication Details

Fetal Diagnosis and Therapy (Clinical Advances and Basic Research)

Vol. 21, No. 5, Year 2006 (Cover Date: August 2006)

Journal Editor: Holzgreve, W. (Basel)
ISSN: 1015–3837 (print), 1421–9964 (Online)

For additional information: http://www.karger.com/FDT


Article / Publication Details

First-Page Preview
Abstract of Paper

Received: 11/17/2004
Accepted: 9/5/2005
Published online: 8/17/2006

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 2

ISSN: 1015-3837 (Print)
eISSN: 1421-9964 (Online)

For additional information: http://www.karger.com/FDT


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