A Simple Parallel Analytical Method of Prenatal ScreeningLi D.a · Yang H.a · Zhang W-H.a · Pan H.a · Wen D.-Q.a · Han F.-C.a · Guo H.-F.a · Wang X.-M.b · Yan X.-J.a
aInstitute of Genetic Diagnosis, School of Pharmacy, State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi’an, and bShenzhen Sino Co., Ltd, Shenzhen, China
Protein microarray has progressed rapidly in the past few years, but it is still hard to popularize it in many developing countries or small hospitals owing to the technical expertise required in practice. We developed a cheap and easy-to-use protein microarray based on dot immunogold filtration assay for parallel analysis of ToRCH-related antibodies including Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus type 1 and 2 in sera of pregnant women. It does not require any expensive instruments and the assay results can be clearly recognized by the naked eye. We analyzed 186 random sera of outpatients at the gynecological department with our microarray and commercial ELISA kit, and the results showed there was no significant difference between the two detection methods. Validated by clinical application, the microarray is easy to use and has a unique advantage in cost and time. It is more suitable for mass prenatal screening or epidemiological screening than the ELISA format.
© 2006 S. Karger AG, Basel
L.D. and Y.H. contributed equally to this work.
Received: March 30, 2005
Accepted after revision: May 3, 2006
Published online: June 21, 2006
Number of Print Pages : 6
Number of Figures : 4, Number of Tables : 2, Number of References : 25
Gynecologic and Obstetric Investigation
Vol. 62, No. 4, Year 2006 (Cover Date: November 2006)
Journal Editor: D'Hooghe, T. (Leuven)
ISSN: 0378–7346 (print), 1423–002X (Online)
For additional information: http://www.karger.com/GOI