Background/Aim: It has been reported that adipose
tissue contain progenitor cells with angiogenic
potential and that therapy based on adipose tissuederived
progenitor cells administration may constitute
a promising cell therapy in patients with ischemic
disease. In this study we evaluated the effect of
culture-expanded mesenchymal stem cells (MSC)
derived from adipose tissue on neovascularization
and blood flow in an animal model of limb ischemia in
immunodeficient mice. Methods: MSC were cultured
from human adipose tissue by collagenase digestion.
Hindlimb ischemia was created by ligating the proximal
femoral artery of male nude mice. Human adipose
tissue stromal cells (hADSC) were transplanted one
day or 7 days after ligation. Results: During culture
expansion of hADSC CD34 expression was
downregulated. The laser Doppler perfusion index
was significantly higher in the CD34(-), Flk-1(-),
CD31(-) ADSC-transplanted group than in the control
group, even when cells were transplanted 7days after
hindlimb ischemia. Histological examination showed
that hADSC transplantation recovered muscle injury
and increased vascular density, compared with the
control group. The effect of hADSC was correlated
with the number of transplanted cells, but not with
the ratio of CD34 expression. In vitro, hADSC can
form vessel-like structure and express von Willibrand
Factor. Conditioned media from hADSC increased
proliferation and inhibited apoptotic cell death in of
human aortic endothelial cells. Conclusion: This study
showed that hADSC can be an ideal source for
therapeutic angiogenesis in ischemic disease.
Copyright / Drug Dosage
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