Journal Mobile Options
Table of Contents
Vol. 114, No. 3-4, 2006
Issue release date: August 2006
Section title: Techniques
Cytogenet Genome Res 114:199–221 (2006)
(DOI:10.1159/000094203)

Spectral karyotyping of human, mouse, rat and ape chromosomes – applications for genetic diagnostics and research

Schrock E. · Zschieschang P. · O’Brien P. · Helmrich A. · Hardt T. · Matthaei A. · Stout-Weider K.
aInstitut für Klinische Genetik, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, Dresden (Germany); bDepartment of Clinical Veterinary Medicine, Centre for Veterinary Science, Veterinary Cytogenetics Group, Madingley Road, Cambridge (UK); cInstitut de Génétique et de Biologie Moléculaire et Cellulaire, CU de Strasbourg, Illkirch (France); dInstitut für Medizinische Genetik, Charité – Universitätsmedizin Berlin, Campus Virchow-Klinikum, Berlin (Germany)

Do you have an account?

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

Register and profit from personalized services (MyKarger) Login Information

Please create your User ID & Password





Contact Information









I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Buy

  • FullText & PDF
  • Unlimited re-access via MyKarger (new!)
  • Unrestricted printing, no saving restrictions for personal use
  • Reduced rates with a PPV account
read more

Direct: USD 38.00
Account: USD 26.50

Select

Rent/Cloud

  • Rent for 48h to view
  • Buy Cloud Access for unlimited viewing via different devices
  • Synchronizing in the ReadCube Cloud
  • Printing and saving restrictions apply

Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe

  • Automatic perpetual access to all articles of the subscribed year(s)
  • Unlimited re-access via Subscriber Login or MyKarger
  • Unrestricted printing, no saving restrictions for personal use
read more

Subcription rates


Select


Article / Publication Details

First-Page Preview
Abstract of Techniques

Received: 5/8/2006
Accepted: 5/19/2006
Published online: 9/1/2006

Number of Print Pages: 23
Number of Figures: 4
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

Spectral karyotyping (SKY) is a widely used methodology to identify genetic aberrations. Multicolor fluorescence in situ hybridization using chromosome painting probes in individual colors for all metaphase chromosomes at once is combined with a unique spectral measurement and analysis system to automatically classify normal and aberrant chromosomes. Based on countless studies and investigations in many laboratories worldwide, numerous new chromosome translocations and other aberrations have been identified in clinical and tumor cytogenetics. Thus, gene identification studies have been facilitated resulting in the dissection of tumor development and progression. For example, different translocation partners of the TEL/ETV6 transcription factor that is specially required for hematopoiesis within the bone marrow were identified. Also, the correct classification of complex karyotypes of solid tumors supports the prognostication of cancer patients. Important accomplishments for patients with genetic diseases, leukemias and lymphomas, mesenchymal tumors and solid cancers are summarized and exemplified. Furthermore, studies of disease mechanisms such as centromeric DNA breakage, DNA double strand break repair, telomere shortening and radiation-induced neoplastic transformation have been accompanied by SKY analyses. Besides the hybridization of human chromosomes, mouse karyotyping has also contributed to the comprehensive characterization of mouse models of human disease and for gene therapy studies.


  

Author Contacts

Request reprints from Evelin Schrock, MD, Professor
Institut für Klinische Genetik, Medizinische Fakultät Carl Gustav Carus
Technische Universität Dresden, Fetscherstraβe 74
DE–01307 Dresden (Germany)
telephone: +49-351-458 5136; fax: +49-351-458 6337
e-mail: evelin.schrock@tu-dresden.de

  

Article Information

This work was supported by the German Federal Ministry of Education and Research, BMBF, BioFuture grant 0311875 (ES).

Manuscript received: 8 May 2006
Accepted in revised form for publication by T. Liehr,: 19 May 2006.
Number of Print Pages : 23
Number of Figures : 4, Number of Tables : 0, Number of References : 192

  

Publication Details

Cytogenetic and Genome Research

Vol. 114, No. 3-4, Year 2006 (Cover Date: August 2006)

Journal Editor: Schmid, M. (Würzburg)
ISSN: 1424–8581 (print), 1424–859X (Online)

For additional information: http://www.karger.com/CGR


Article / Publication Details

First-Page Preview
Abstract of Techniques

Received: 5/8/2006
Accepted: 5/19/2006
Published online: 9/1/2006

Number of Print Pages: 23
Number of Figures: 4
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.