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Table of Contents
Vol. 220, No. 5, 2006
Issue release date: September 2006
Section title: Original Paper
Ophthalmologica 2006;220:317–322
(DOI:10.1159/000094622)

Effect of High Glucose Concentration on Corneal Collagen Biosynthesis

Robert L. · Menasche M. · Robert A.M. · Renard G.
Laboratoire de Recherche Ophtalmologique, Hôpital Hôtel-Dieu, Paris, France

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: December 22, 2005
Accepted: December 29, 2005
Published online: September 07, 2006
Issue release date: September 2006

Number of Print Pages: 6
Number of Figures: 6
Number of Tables: 2

ISSN: 0030-3755 (Print)
eISSN: 1423-0267 (Online)

For additional information: http://www.karger.com/OPH

Abstract

The effect of high glucose concentration (3 g/l) on bovine corneal total protein and collagen biosynthesis was studied, using 3H-proline incorporation in explant cultures with protein and collagen determinations. The high glucose concentration increased the incorporation of 3H-proline in total corneal proteins as well as in collagens. The specific radioactivity of stromal collagens was strongly increased in these conditions. Mannitol was used to control the osmotic effect of the high glucose concentration, both at 1 and 3 g/l concentrations. Mannitol did not increase the incorporation of 3H-proline in total proteins or collagens, but on the contrary decreased it. The high glucose concentration decreased the excretion of neosynthesized proteins and collagens in the culture medium, but did not affect the total protein or collagen content of the corneas. The strong increase in the specific radioactivity of corneal collagens in the presence of 3 g/l glucose suggests an increased turnover of collagens in diabetic corneas. The increased biosynthesis of collagens together with their decreased elimination in the extracellular compartment can create the conditions for the formation and accumulation of advanced glycation endproducts by the Maillard reaction. This can induce and stimulate the liquefaction of the vitreous body leading to sight-threatening disorders such as diabetic retinopathy, retinal detachment, glaucoma, cataract formation and age-related macular degeneration.

© 2006 S. Karger AG, Basel


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: December 22, 2005
Accepted: December 29, 2005
Published online: September 07, 2006
Issue release date: September 2006

Number of Print Pages: 6
Number of Figures: 6
Number of Tables: 2

ISSN: 0030-3755 (Print)
eISSN: 1423-0267 (Online)

For additional information: http://www.karger.com/OPH


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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