Investigation of Potential Antibacterial Properties of Methanol Extracts from Fungus Ganoderma applanatumMoradali M.-F.a · Mostafavi H.b · Hejaroude G.-A.a · Tehrani A.S.a · Abbasi M.c · Ghods S.a
aPlant Protection Department, Faculty of Agriculture, Tehran University, Karaj, bOrganic Chemistry and Biochemistry Department, Faculty of Chemistry, Tabriz University, Tabriz, cPlant Pest and Diseases Research Institute, Tehran, Iran
In this study we searched for antibacterial compounds in methanol extracts of three layers (tube layer, context and cutis) of Ganoderma applanatum fruit body against Bacillus subtilis ATCC 6051 as a gram-positive bacterium and Escherichia coli ATCC 25922 and Pseudomonas syringae pv. syringae DPIC 219 as gram-negative bacteria. We found antibacterial properties in all three layers only against gram-negative bacteria. The fractions were isolated and purified by thin layer chromatography (TLC) and high-performance liquid chromatography. After purification, four major compounds – A, B, C and D – were tested for antibacterial properties, and B and D were found to possess antibacterial properties. The results of TLC and spectral data, including mass spectra and 1H NMR of compounds B and C, showed that they were saturated fatty acids and compound D was palmitic acid (hexadecanoic acid) (16:0).
© 2006 S. Karger AG, Basel
Presented at the 1st World Conference on Magic Bullets – EHRLICH 2004, Nürnberg, Germany, September 9–11, 2004.
Received: October 29, 2004
Accepted after revision: September 6, 2005
Published online: August 4, 2006
Number of Print Pages : 4
Number of Figures : 0, Number of Tables : 4, Number of References : 5
Chemotherapy (International Journal of Experimental and Clinical Chemotherapy)
Vol. 52, No. 5, Year 2006 (Cover Date: August 2006)
Journal Editor: Sörgel, F. (Nürnberg-Heroldsberg)
ISSN: 0009–3157 (print), 1421–9794 (Online)
For additional information: http://www.karger.com/CHE