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Vol. 78, No. 2, 2006
Issue release date: October 2006
Section title: Original Paper
Pharmacology 2006;78:72–80
(DOI:10.1159/000095637)

Evidence for the Presence of Histamine Uptake into the Synaptosomes of Rat Brain

Sakurai E. · Sakurai E. · Oreland L. · Nishiyama S. · Kato M. · Watanabe T. · Yanai K.
aDepartment of Pharmacology, Tohoku University School of Medicine, Sendai, bDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, Aomori University, Aomori, Japan; and cDepartment of Medical Pharmacology, Uppsala University, Uppsala, Sweden

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/14/2005
Accepted: 7/14/2006
Published online: 10/4/2006

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 3

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: http://www.karger.com/PHA

Abstract

Histamine has many physiological roles in the brain and periphery. Neuronal histamine is metabolized almost exclusively by histamine N-methyltransferase. Although several neurotransmitter systems such as dopamine and 5-hydroxytryptamine have their specific reuptake system in their neurons and glial cells, a specific histamine reuptake system into the corresponding nerve terminals or glial cells has not yet been clearly elucidated. We characterized the uptake of histamine into the P2 fractions of rat brain homogenized in 0.32 mol/l sucrose using in vitro uptake techniques. [3H]histamine uptake increased with the increment of added protein amount and elapsed time. [3H]histamine uptake was also temperature-dependent. The uptake of [3H]histamine into the P2 fractions occurs by two saturable processes, a high-affinity and a low-affinity, characterized by Km values of 0.16 and 1.2 µmol/l, respectively. Na+, Cl and HCO3 ions were essential for the uptake of histamine in P2 fractions. [3H]histamine uptake was inhibited in the presence of several tricyclic antidepressants. In accordance with this, the endogenous release of histamine from brain slices evoked by 100 mmol/l K+ was augmented in the presence of 20 µmol/l imipramine. These results further support the existence of a specific histamine uptake system in the brain, although the precise molecular entities have not been identified until now.


  

Author Contacts

Prof. Kazuhiko Yanai, MD, PhD, Department of Pharmacology
Tohoku University School of Medicine
2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575 (Japan)
Tel. +81 22 717 8055, Fax +81 22 717 8060
E-Mail yanai@mail.tains.tohoku.ac.jp

  

Article Information

Received: July 14, 2005
Accepted after revision: July 14, 2006
Published online: September 7, 2006
Number of Print Pages : 9
Number of Figures : 5, Number of Tables : 3, Number of References : 48

  

Publication Details

Pharmacology (International Journal of Experimental and Clinical Pharmacology)

Vol. 78, No. 2, Year 2006 (Cover Date: October 2006)

Journal Editor: Donnerer, J. (Graz)
ISSN: 0031–7012 (print), 1423–0313 (Online)

For additional information: http://www.karger.com/PHA


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 7/14/2005
Accepted: 7/14/2006
Published online: 10/4/2006

Number of Print Pages: 9
Number of Figures: 5
Number of Tables: 3

ISSN: 0031-7012 (Print)
eISSN: 1423-0313 (Online)

For additional information: http://www.karger.com/PHA


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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