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Vol. 50, No. 1, 2007
Issue release date: December 2006
Section title: Original Paper
Intervirology 2007;50:45–51
(DOI:10.1159/000096312)

A New Algorithm for Deduction of Hepatitis B Surface Antigen Subtype Determinants from the Amino Acid Sequence

Purdy M.A.a · Talekar G.a · Swenson P.b, c · Araujo A.a · Fields H.a
aDivision of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Ga., bPublic Health – Seattle & King County, and cDepartment of Laboratory Medicine, University of Washington, Seattle, Wash., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 11/24/2006
Issue release date: December 2006

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 4

ISSN: 0300-5526 (Print)
eISSN: 1423-0100 (Online)

For additional information: http://www.karger.com/INT

Abstract

Objective: We have reexamined hepatitis B virus subtypes to determine the role of specific HBsAg amino acids in serologic reactivity because of problematic genotype/subtype associations seen in a set of geographically diverse serum specimens. Methods: We obtained DNA sequences for 491 HBsAg-positive specimens from geographically distinct locations, determined their genotypes through phylogenetic analysis, and subtyped the specimens using an algorithm derived from published data on the molecular basis of HBsAg subtype reactivity. Problematic samples were subtyped serologically to resolve conflicts based on the amino acid sequence alone. Results: Three isolates were found to have unusual genotype/subtype associations. Examination of the isolates’ amino acid sequences suggested amino acid positions 122, 127, 140, 159 and 160 can be used to determine subtype reactivity from HBsAg amino acid sequences, while position 134, previously thought to play a role, is no longer important. Conclusions: This re-examination of hepatitis B virus subtypes shows the involvement of amino acid positions 122, 127, 140, 159 and 160 in HBsAg reactivity. While d, y, and r reactivities are controlled by single amino acid changes, w reactivity is determined by positions 122, 127, 140, and 159.

© 2007 S. Karger AG, Basel


  

Author Contacts

Michael A. Purdy
Centers for Disease Control and Prevention
MS-A33, 1600 Clifton Rd. NE
Atlanta, GA 30333 (USA)
Tel. +1 404 639 2332, Fax +1 404 639 1563, E-Mail MPurdy@cdc.gov

  

Article Information

This informaton is distributed solely for the purpose of predissemination peer review under applicable information quality guidelines. It has not been formally disseminated by the Centers for Disease Control and Prevention/the Agency for Toxic Substances and Disease Registry. It does not represent and should not be construed to represent any agency determination or policy.

Received: September 23, 2005
Accepted after revision: February 28, 2006
Published online: November 24, 2006
Number of Print Pages : 7
Number of Figures : 3, Number of Tables : 4, Number of References : 27

  

Publication Details

Intervirology (International Journal of Basic and Medical Virology)

Vol. 50, No. 1, Year 2007 (Cover Date: December 2006)

Journal Editor: Liebert, U.G. (Leipzig)
ISSN: 0300–5526 (print), 1423–0100 (Online)

For additional information: http://www.karger.com/INT


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 11/24/2006
Issue release date: December 2006

Number of Print Pages: 7
Number of Figures: 3
Number of Tables: 4

ISSN: 0300-5526 (Print)
eISSN: 1423-0100 (Online)

For additional information: http://www.karger.com/INT


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