Structure and genetic mapping of the Cytochrome P450 gene (CYP1A5) in the turkey (Meleagris gallopavo)Reed K.M. · Mendoza K.M. · Coulombe Jr R.A.
aDepartment of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN, and bDepartment of Veterinary Sciences, Utah State University, Logan, UT (USA)
Cytochromes P450 (P450) are a superfamily of membrane-bound hemoproteins that oxidize a large number of endogenous and exogenous compounds. The recently cloned P450 gene (CYP1A5) encodes the primary protein responsible for epoxidation of aflatoxin B1 (AFB1) in the turkey, an animal extremely sensitive to this mycotoxin. Hypersensitivity of turkeys to AFB1 was first demonstrated by association with ‘Turkey X Disease’ which caused widespread deaths of turkeys and other poultry throughout Europe in the 1960s, later shown to be caused by AFB1-contaminated feed. In this study, comparative genomic approaches were used to selectively amplify and sequence the introns and 3′ flanking region of CYP1A5. The structure of the CYP1A5 gene in the turkey is shown to be equivalent to that of the human CYP1A genes with seven exons of 38, 858, 127, 90, 124, 87 and 307 bp, respectively, and six introns. A single nucleotide polymorphism (SNP) in the 3′ UTR was used to assign CYP1A5 to turkey linkage group M16 (equivalent to chicken chromosome 10). The results of this study provide the framework for identifying allelic variants of this biochemically important P450 gene in poultry.
Request reprints from Kent M. Reed
Department of Veterinary and Biomedical Sciences
College of Veterinary Medicine
295 Animal Science/Veterinary Medicine, 1988 Fitch Avenue
University of Minnesota, St. Paul, MN 55108 (USA)
telephone: +1 612 624 1287; fax: +1 612 625 0204
The authors wish to acknowledge support of NRI competitive grants 2002-35204-12294, and 2004-35205-14217, from USDA-CSREES, and support from the Utah and Minnesota Agricultural Experiment Stations.
Manuscript received: 17 April 2006
Accepted in revised form for publication by M. Schmid,: 3 July 2006.
Number of Print Pages : 6
Number of Figures : 3, Number of Tables : 1, Number of References : 18
Cytogenetic and Genome Research
Vol. 116, No. 1-2, Year 2007 (Cover Date: January 2007)
Journal Editor: Schmid, M. (Würzburg)
ISSN: 1424–8581 (print), 1424–859X (Online)
For additional information: http://www.karger.com/CGR