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Vol. 19, No. 1-4, 2007
Issue release date: 2007
Section title: Original Paper
Cell Physiol Biochem 2006;19:001–008
(DOI:10.1159/000099187)

Direct Mechano-Stress Sensitivity of TRPM7 Channel

Numata T.1,2,3 · Shimizu T.1,2 · Okada Y.1,2
1Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki,2Department of Physiological Sciences, School of Life Science, The Graduate University for Advanced Studies (Sokendai), Okazaki,3Japan Society for the Promotion of Science, Tokyo

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Abstract

It has recently been shown that shear stress augments the heterologously expressed TRPM7 channel activity by exocytosis-mediated incorporation of TRPM7 into the plasma membrane. On the other hand, our recent study has shown that the TRPM7-like channel endogenously expressed in HeLa cells is activated by membrane expansion induced by membrane stretch or osmotic cell swelling. Thus, the present study was aimed at exploring the possibility that the heterogously expressed TRPM7 channel is activated directly by membrane expansion in a manner independent of exocytosis. Here, whole-cell currents of the TRPM7 channel heterologously expressed in HEK293T cells were found to be augmented not only by perfusion of bath solution but also by osmotic swelling even under the conditions where exocytotic events can hardly take place in the cytosol dialyzed with ATP-free, Ca2+-free and EGTA-containing pipette solution. In addition, shear stress-induced augmentation was not affected by a blocker of vesicular protein traffic, brefeldin A. Furthermore, in cell-free patches, membrane stretch directly augmented single-channel activity of TRPM7 by increasing Po value at ? 20mV. We thus conclude that the TRPM7 channel can be directly activated by mechano-stress in a manner independent of exocytosis-mediated incorporation of this channel protein into the plasma membrane.

© 2007 S. Karger AG, Basel


  

Author Contacts

Ph D MD Yasunobu Okada
Departement of Cell Physiology, National Institute for Physiological Sciences
Okazaki 444-8585 (Japan)
Tel. +81 564 55 7731, Fax: +81 564 55 7735
E-Mail okada@nips.ac.jp

  

Article Information

Accepted: October 16, 2006
Number of Print Pages : 8

  

Publication Details

Cellular Physiology and Biochemistry (International Journal of Experimental Cellular Physiology, Biochemistry andPharmacology)

Vol. 19, No. 1-4, Year 2007 (Cover Date: 2007)

Journal Editor: F. Lang, Tübingen
ISSN: 1015–8987 (print), 1421–9778 (Online)

For additional information: http://www.karger.com/journals/cpb


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 1/16/2007
Issue release date: 2007

Number of Print Pages: 8
Number of Figures: 0
Number of Tables: 0

ISSN: 1015-8987 (Print)
eISSN: 1421-9778 (Online)

For additional information: http://www.karger.com/CPB


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