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Vol. 92, No. 2, 2007
Issue release date: August 2007
Section title: Original Paper
Neonatology 2007;92:96–104
(DOI:10.1159/000100808)

Antiviral Activity of Purified Human Breast Milk Mucin

Habte H.H. · Kotwal G.J. · Lotz Z.E. · Tyler M.G. · Abrahams M. · Rodriques J. · Kahn D. · Mall A.S.
Departments of aSurgery, bMedical Virology and cMolecular and Cell Biology, University of Cape Town, Cape Town, South Africa

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/3/2006
Accepted: 12/6/2006
Published online: 3/14/2007

Number of Print Pages: 9
Number of Figures: 9
Number of Tables: 1

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO

Abstract

Human breast milk is known to contain numerous biologically active components which protect breast fed infants against microbes, viruses, and toxins. The purpose of this study was to purify and characterize the breast milk mucin and determine its anti-poxvirus activity. In this study human milk mucin, free of contaminant protein and of sufficient quantity for further analysis, was isolated and purified by Sepharose CL-4B gel filtration and cesiumchloride density-gradient centrifugation. Based on the criteria of size and appearance of the bands and their electrophoretic mobility on sodium dodecyl sulfate polyacrylamide-gel electrophoresis, Western blotting together with the amino acid analysis, it is very likely that the human breast milk mucin is MUC1. It was shown that this breast milk mucin inhibits poxvirus activity by 100% using an inhibition assay with a viral concentration of 2.4 million plaque-forming units/ml. As the milk mucin seems to aggregate poxviruses prior to their entry into host cells, it is possible that this mucin may also inhibit other enveloped viruses such as HIV from entry into host cells.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 6/3/2006
Accepted: 12/6/2006
Published online: 3/14/2007

Number of Print Pages: 9
Number of Figures: 9
Number of Tables: 1

ISSN: 1661-7800 (Print)
eISSN: 1661-7819 (Online)

For additional information: http://www.karger.com/NEO


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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Naarding MA, Ludwig IS, Groot F, Berkhout B, Geijtenbeek TBH, Pollakis G, Paxton WA: Lewis X component in human milk binds DC-SIGN and inhibits HIV-1 transfer to CD4+ T lymphocytes. J Clin Invest 2005;115:3256–3264.
  2. Schroten H, Hanisch FG, Plogmann R, Hacker J, Uhlenbruck G, Nobis-Bosch R, Wahn V: Inhibition of adhesion of S-fimbriated Escherichia coli to buccal epithelial cells by human milk fat globule membrane components: a novel aspect of the protective function of mucins in the nonimmunoglobulin fraction. Infect Immun 1992;60:2893–2899.
  3. Wiederschain GY, Newburg DS: Glycoconjugate stability in human milk: glycosidase activities and sugar release. J Nutr Biochem 2001;12:559–564.
  4. Yolken RH, Peterson JA, Vonderfecht SL, Fouts ET, Midthun K, Newburg DS: Human milk inhibits rotavirus replication and prevents experimental gastroenteritis. J Clin Invest 1992;90:1984–1991.
  5. Duwe AK, Ceriani RL: Human milk-fat globule membrane derived mucin is a disulfide-linked heteromer. Biochem Biophys Res Commun 1989;165:1305–1311.
  6. Hanisch F-G, Uhlenbruck G, Peter-Katalinic J, Egge H, Dabrowski J, Dabrowski U: Structures of neutral O-linked polylactosaminoglycans on human skim mucins. J Biol Chem 1989;264:872–883.
  7. Newburg DS, Linhardt RJ, Ampofo SA, Yolken RH: Human milk glycosaminoglycans inhibit HIV glycoprotein gp120 binding to its host cell CD4 receptor. J Nutr 1994;125:419–424.
  8. Patton S, Gendler SJ, Spicer AP: The epithelial mucin, MUC1, of milk, mammary gland and other tissues. Biochim Biophys Acta 1995;1241:407–424.
  9. Imam A, Laurence DJR, Neville AM: Isolation and characterization of two individual glycoprotein components from human milk-fat-globule membranes. Biochem J 1982;207:37–41.
  10. Keenan TW, Morre DJ, Olson DE, Yunghans WN, Patton S: Biochemical and morphological comparison of plasma membrane and milk fat globule membrane from bovine mammary gland. J Cell Biol 1970;44:80–93.
  11. Peterson JA, Patton S, Hamosh M: Glycoproteins of human milk fat globule in the protection of the breast-fed infant against infections. Biol Neonate 1998;74:143–162.
  12. Shimizu M, Yamauchi K: Isolation and characterization of mucin-like glycoprotein in human milk fat globule membrane. J Biochem 1982;91:515–524.
  13. Imam A, Laurence DJR, Neville AM: Isolation and characterization of a major glycoprotein from milk-fat-globule membrane of human breast milk. Biochem J 1981;193:47–54.
  14. Pallesen LT, Andersen MH, Nielsen RL, Berglund L, Petersen TE, Rasmussen LK, Rasmussen JT: Purification of MUC1 from bovine milk-fat globules and characterization of a corresponding full-length cDNA clone. J Dairy Sci 2001;84:2591–2598.
  15. Patton S: Some practical implications of the milk mucins. J Dairy Sci 1999;82:1115–1117.
  16. Shimizu M, Yamauchi K, Miyauchi Y, Sakurai T, Tokugawa K, Mcilhinney RAJ: High-Mr glycoprotein profile in human milk serum and fat-globule membrane. Biochem J 1986;233:725–730.
  17. Newburg DS: Human milk glycolconjugates that inhibit pathogens. Curr Med Chem 1999;6:117–127.
  18. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680–685.
  19. Creeth JM, Denborough MA: The use of equilibrium-density-gradient methods for the preparation and characterization of blood-group-specific glycoproteins. Biochem J 1970;117:879–891.
  20. Klapper DG: A new low cost, fully automated amino acid analyzer; in Elzinga M (ed): Methods in Protein Sequence Analysis. Clifton New Jersey, Humana Press, 1982, pp 509–517.
  21. Cohen AS, Strydom DJ: Amino acid analysis utilizing phenylisothiocyanate derivatives. Anal Biochem 1988;174:1–16.
  22. Carlstedt I, Sheehan J, Ulmsten U, Wingerup L: Isolation and purification of the mucin component of human cervical mucus. Adv Exp Med Biol 1982;144:273–275.
  23. Allen A: Structure and function of gastrointestinal mucus; in Johnson LR (ed): Physiology of the Gastrointestinal Tract. New York, Raven Press, 1981, Chapter 19, pp 359–382.
  24. Mall AS: Gastro-duodenal mucin isolation and structure. PhD thesis. University of Newcastle upon Tyne, 1988.
  25. Mall AS, McLeod HA, Hickman R, Kahn D, Dent DM: Fragmentation pattern of mucins in normal and diseased gastric mucosae: a glycoprotein fractionates with gastric mucins purified from mucosal scrapings of cancer and peptic ulcer patients. Digestion 1999;60:216–226.
  26. Newburg DS, Viscidi RP, Ruff A, Yolken RH: A human milk factor inhibits binding of human immunodeficiency virus to the CD4 receptor. Pediatr Res 1992;31:22–28.
  27. Billings B, Smith SA, Zhang Z, Lahiri DK, Kotwal GJ: Lack of N1L gene expression results in a significant decrease of vaccinia virus replication in mouse brain. Ann NY Acad Sci 2004;1030:297–302.
  28. Kotwal GJ, Kaczmarek JN, Leivers S, Ghebremariam YT, Kulkarni AP, Bauer G, De Beer C, Preiser W, Rahman Mohamed A: Anti-HIV, anti-Poxvirus, and Anti-SARS activity of nontoxic, acidic plant extract from the Trifollium species secomet-V/anti-vac suggests that it contains a novel broad-spectrum antiviral. Ann N Y Acad Sci 2004;1056:293–302.

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