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Vol. 51, No. 3, 2007
Issue release date: August 2007
Section title: Original Paper
Ann Nutr Metab 2007;51:244–251
(DOI:10.1159/000104144)

Human Gastric Juice Contains Chitinase That Can Degrade Chitin

Paoletti M.G. · Norberto L. · Damini R. · Musumeci S.
aDepartment of Biology, Laboratory Agroecology and Ethnobiology, and bDepartment of Surgical and Gastroenterological Science, Section of 1st General Surgery, Surgical Endoscopy Unit, University of Padova, Padova, cDepartment of Pharmacology, Gynecology and Obstetrics, Pediatrics, University of Sassari and Institute of Biomolecular Chemistry, CNR, Li Punti (SS), Italy

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 11/7/2006
Accepted: 3/9/2007
Published online: 6/18/2007

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 2

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: http://www.karger.com/ANM

Abstract

Chitin digestion by humans has generally been questioned or denied. Only recently chitinases have been found in several human tissues and their role has been associated with defense against parasite infections and to some allergic conditions. In this pilot study we tested the gastric juices of 25 Italian subjects on the artificial substrates 4-methylumbelliferyl-β-D-N,N’,diacetylchitobiose or/and fluorescein isothiocyanate (FITC) chitin to demonstrate the presence of a chitinase activity. Since this chitinase activity was demonstrated at acidic pH, it is currently referred to acidic mammalian chitinase (AMCase). AMCase activity was present in gastric juices of twenty of 25 Italian patients in a range of activity from 0.21 to 36.27 nmol/ml/h and from 8,881 to 1,254,782 fluorescence emission (CPS), according to the used methods. In the remaining five of 25 gastric juices, AMCase activity was almost absent in both assay methods. An allosamidine inhibition test and the measurement at different pH values confirmed that this activity was characteristic of AMCase. The absence of activity in 20% of the gastric juices may be a consequence of virtual absence of chitinous food in the Western diet.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 11/7/2006
Accepted: 3/9/2007
Published online: 6/18/2007

Number of Print Pages: 8
Number of Figures: 3
Number of Tables: 2

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: http://www.karger.com/ANM


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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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