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Vol. 7, No. 2-3, 2007
Issue release date: July 2007
Pancreatology 2007;7:174–179

Investigation of Relaxant Effects of Propofol on Sheep Sphincter of Oddi

Bagcivan I. · Gursoy S. · Yildirim M.K. · Kaya Temiz T. · Yildirim S. · Yilmaz A. · Turan M.
Departments of aPharmacology, bAnesthesiology, cInternal Medicine, Cumhuriyet University School of Medicine, Sivas, and dGerman Hospital (Universal Hospitals Group), Department of General Surgery, Istanbul, Turkey

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Background/Aims: Intravenous anesthetics are often used for conscious sedation in endoscopic retrograde cholangiopancreatography (ERCP) and endoscopic sphincter of Oddi (SO) manometry. This study was designed to investigate the effects of propofol on sheep SO. Methods: SO rings were mounted in a tissue bath and tested for changes in isometric tension in response to propofol (10–8–10–4M) in the presence or absence of L-NAME (3 × 10–5M), a non-specific inhibitor of nitric oxide (NO) synthase; indomethacin (10–5M), an inhibitor of cyclooxygenase; glibenclamide (10–5M), an inhibitor of ATP-sensitive potassium channels; tetraethylammonium (3 × 10–4M), inhibitors of calcium-activated potassium channels; 4-aminopyridine (10–3M), a voltage-dependent potassium channel blocker. Furthermore, we investigated the Ca2+ antagonist feature of propofol in precontracted SO rings by CaCl2. Results: Carbachol (10–9–10–5M) induced concentration-dependent contraction responses in the SO rings. Propofol (10–8–10–4M) produced concentration-dependent relaxation on isolated SO rings precontracted by carbachol (10–6M). Preincubation of SO rings by L-NAME (3 × 10–5M), indomethacin (10–5M), glibenclamide (10–5M), and 4-aminopyridine (10–3M) did not produce a significant alteration on propofol-induced relaxation responses (p > 0.05), while preincubation by tetraethylammonium (3 × 10–4M) significantly decreased the propofol-induced relaxation responses (p < 0.05). Propofol (10–8–10–4M) induced concentration-dependently relaxations in precontracted isolated SO rings by CaCl2. Conclusion: The results suggest that propofol induced concentration-dependent relaxations in precontracted isolated SO rings. These relaxations are independent from NO, cyclooxygenase metabolites, and opened ATP-sensitive and voltage-dependent potassium channels. Opened Ca2+-sensitive K+ channels and inhibited L-type Ca2+ channels existing in smooth muscle by propofol can contribute to these relaxations. Propofol can be beneficial as alternative drugs for obtaining selective relaxation during SO manometry after controlled clinical studies.

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  1. Staritz M: Pharmacology of the sphincter of Oddi. Endoscopy 1988;20:171–174.
  2. Toouli J, Baker RA: Innervation of the sphincter of Oddi: physiology and considerations of pharmacological intervention in biliary dyskinesia. Pharmacol Ther 1991;49:269–281.
  3. Padbury RT, Furness JB, Baker RA, Toouli J, Messenger JP: Projections of nerve cells from the duodenum to the sphincter of Oddi and gallbladder of the Australian possum. Gastroenterology 1993;104:130–136.
  4. Saccone GTP, Harvey JR, Baker RA, Toouli J: Intramural neural pathways between the duodenum and sphincter of Oddi in the Australian brush-tailed possum in vivo. J Physiol 1994;481:447–456.
  5. Simula ME, Harvey JR, Costi D, Baker RA, Toouli J, Saccone G: In vitro characterisation of intramural neural pathway between the duodenum and sphincter of Oddi in the Australian brush-tailed possum. J Auton Nerv Syst 1997;63:77–84.
  6. Classen M, Demling L: Endoscopic sphincterotomy of the papilla of Vater and extraction of stones from the choledochal duct. Dtsch Med Wochenschr 1974;99:496–497.
  7. Kawai K, Akasaka Y, Murakami K, Tada M, Koli Y; Endoscopic sphincterotomy of the ampulla of Vater. Gastrointest Endosc 1974;4:148–151.
  8. Freeman ML: Understanding risk factors and avoiding complications with endoscopic retrograde cholangiopancreatography. Curr Gastroenterol Rep 2003;5:145–153.
  9. Cohen J, Haber GB, Dorais JA, Scheider DM, Kandel GP, Kortan PP, Marcon NE: A randomized double-blind study of the use of droperidol for conscious sedation during therapeutic endoscopy in difficult to sedate patients. Gastrointest Endosc 2000;51:546–551.
  10. Hubbard GP, Wolfe KR: Meperidine misuse in a patient with sphincter of Oddi dysfunction. Ann Pharmacother 2003;37:534–537.
  11. McCammon RL, Stoelting RK, Madura JA: Effects of butorphanol, nalbuphine, and fentanyl on intrabiliary tract dynamics. Anesth Analg 1984;63:139–142.
  12. Turan M, Bagcivan I, Gursoy S, Sarac B, Duman M, Kaya T: In vitro effects of intravenous anesthetics on the sphincter of Oddi strips of sheep. Pancreatology 2005;5:215–219.
  13. Weir SW, Weston AH: The effects of BRL 34915 and nicorandil on electrical and mechanical activity and on 86Rb efflux in rat blood vessels. Br J Pharmacol 1986;88:121–128.
  14. Arrowsmith JB, Gerstman BB, Flischer DE, et al: Results from the American Society for Gastrointestinal Endoscopy/US Food and Drug Administration collaborative study on complication rates and drug use during gastrointestinal endoscopy. Gastrointest Endosc 1991;37:421–427.
  15. Economou G, Ward-McQuaid JN: A crossover comparison of the effect of morphine, pethidine, pentazocine, and phenazocine on biliary pressure. Gut 1971;12:218–221.
  16. Amrein R, Hetzel W, Allen SR. Co-induction of sedation: the rationale. Eur J Anaesth 1995;12(suppl):3–9.
  17. Godsiff L, Magee L, Park GR. Propofol versus propofol with midazolam for laryngeal mask insertion. Eur J Anaesth 1995;12(suppl):35–40.
  18. Goff JS: Effect of propofol on human sphincter of Oddi. Dig Dis Sci 1995;40:2364–2367.
  19. Allescher HD: How to sedate for endoscopic sphincter of Oddi manometry? Endoscopy 1993;25:399–400.
  20. Baron TH, Dalton CB, Cotton PB, May GR, Milton LG, Chari RS, Meyers WC: The effect of propofol on the canine sphincter of Oddi. HPB Surg 1994;7:297–304.
  21. Wehrmann T, Kokabpick S, Lembcke B, Caspary W. F, Seifert H. Efficacy and safety of intravenous propofol sedation during routine ERCP: a prospective, controlled study. Gastrointest Endosc 1999;49:677–683.
  22. Bult H, Boeckxtaens GE, Pelckmans PA, Jordaens FH, Van Maercke YM, Herman AG: Nitric oxide as an inhibitory non-adrenergic non-cholinergic neurotransmitter. Nature 1990;345:346–347.
  23. Stark ME, Szurszewski JH: The role of nitric oxide in gastrointestinal and hepatic function and disease. Gastroenterology 1991;103:1928–1949.
  24. Brayden JE: Potassium channels in vascular smooth muscle. Clin Exp Pharmacol Physiol 1996;23:1069–1076.

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