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Vol. 187, No. 3, 2008
Issue release date: March 2008
Cells Tissues Organs 2008;187:177–185

In vivo Adipose Tissue Regeneration by Adipose-Derived Stromal Cells Isolated from GFP Transgenic Mice

Mizuno H. · Itoi Y. · Kawahara S. · Ogawa R. · Akaishi S. · Hyakusoku H.
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We have previously demonstrated that pluripotent stem cells can be obtained from green fluorescence protein (GFP) transgenic mouse adipose tissue. In this study, we sought to determine whether adipose tissue regeneration can be induced in vivo using adipose-derived stromal cells (ASCs) from GFP mice. ASCs were isolated from inguinal fat pads of GFP mice, as described in our previous publication. After incubation in two passages in the control medium, the cells were incubated in the induction medium to induce adipogenesis. Induced ASCs were merged with fibrin glue, and the mixture was injected subcutaneously into the dorsum of athymic mice. Finally, specimens were harvested and analyzed morphologically and histologically. The regenerated tissue was macroscopically semitransparent and soft with slight angiogenesis. Fluorescence microscopy revealed that the specimens strongly emitted green fluorescence, suggesting that the transplanted ASCs had contributed to adipogenesis. Both hematoxylin and eosin and oil red O staining revealed that cells containing small lipid droplets had been regenerated histologically. These findings suggest that ASCs could contribute to adipose tissue regeneration in vivo. ASCs may be an ideal source for adipose tissue regeneration, which may in turn play an important role in augmentation surgery in surgically treated cancer or trauma patients.

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  1. Asakura, A., M. Komaki, M. Rudnicki (2001) Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation. Differentiation 68: 245–253.
  2. Ashjian, P.H., A.S. Elbarbary, B. Edmonds, D. DeUgarte, M. Zhu, P.A. Zuk, H.P. Lorenz, P. Benhaim, M.H. Hedrick (2003) In vitro differentiation of human processed lipoaspirate cells into early neural progenitors. Plast Reconstr Surg 111: 1922–1931.
  3. Bach, A.D., H. Bannasch, T.J. Galla, K.M. Bittner, G.B. Stark (2001) Fibrin glue as matrix for cultured autologous urothelial cells in urethral reconstruction. Tissue Eng 7: 45–53.
  4. Beahm, E.K., R.L. Walton, C.W. Patrick, Jr. (2003) Progress in adipose tissue construct development. Clin Plast Surg 30: 547– 558.
  5. Clavijo-Alvarez, J.A., J.P. Rubin, J. Bennett, V.T. Nguyen, J. Duras, C. Underwood, K.G. Marra (2006) A novel perfluoroelastomer seeded with adipose-derived stem cells for soft tissue repair. Plast Reconstr Surg 118: 1132–1142.
  6. De Bari, C., F. Dell’Accio, F.P. Luyten (2001a) Human periosteum-derived cells maintain phenotypic stability and chondrogenic potential throughout expansion regardless of donor age. Arthritis Rheum 44: 85–95.
  7. De Bari, C., F. Dell’Accio, P. Tylzanowski, F.P. Luyten (2001b) Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum 44: 1928–1942.
  8. Dinsmore, J., J. Ratliff, T. Deacon, P. Pakzaban, D. Jacoby, W. Galpern, O. Isacson (1996) Embryonic stem cells differentiated in vitro as a novel source of cells for transplantation. Cell Transplant 5: 131–143.
  9. Dragoo, J.L., J.Y. Choi, J.R. Lieberman, J. Huang, P.A. Zuk, J. Zhang, M.H. Hedrick, P. Benhaim (2003) Bone induction by BMP-2 transduced stem cells derived from human fat. J Orthop Res 21: 622–629.
  10. Ellenbogen, R. (1986) Free autogenous pearl fat grafts in the face – a preliminary report of a rediscovered technique. Ann Plast Surg 16: 179–194.
  11. Ersek, R.A. (1991) Transplantation of purified autologous fat: a 3-year follow-up is disappointing. Plast Reconstr Surg 87: 219–227.
  12. Halberstadt, C., C. Austin, J. Rowley, C. Culberson, A. Loebsack, S. Wyatt, S. Coleman, L. Blacksten, K. Burg, D. Mooney, W. Holder, Jr. (2002) A hydrogel material for plastic and reconstructive applications injected into the subcutaneous space of a sheep. Tissue Eng 8: 309–319.
  13. Hartrampf, C.R., M. Scheflan, P.W. Black (1982) Breast reconstruction with a transverse abdominal island flap. Plast Reconstr Surg 69: 216–225.
  14. Hong, L., I.A. Peptan, A. Colpan, J.L. Daw (2006) Adipose tissue engineering by human adipose-derived stromal cells. Cells Tissues Organs 183: 133–140.
  15. Horch, R., H. Bannasch, J. Kopp, C. Andree, G. Stark (1998) Single-cell suspensions of cultured human keratinocytes in fibrin-glue reconstitute of the dermis. Cell Transplant 7: 309–317.
  16. Huang, J.I., P.A. Zuk, N.F. Jones, M. Zhu, M. Hedrick, P. Benhaim (2004) Chondrogenic potential of multipotential cells from human adipose tissue. Plast Reconstr Surg 113: 585–594.
  17. Isogai, N., W.J. Landis, R. Mori, Y. Gotoh, L.C. Gerstenfeld, J. Upton, J.P. Vacanti (2000) Experimental use of fibrin glue to induce site-directed osteogenesis from cultured periosteal cells. Plast Reconstr Surg 105: 953–963.
  18. Juhasz, I., G.F. Murphy, H.C. Yan, M. Herlyn, S.M. Albelda (1993) Regulation of extracellular matrix proteins and integrin cell substratum adhesion receptors on epithelium during cutaneous human wound healing in vivo. Am J Pathol 143: 1458–1469.
  19. Kimura, Y., M. Ozeki, T. Inamoto, Y. Tabata (2003) Adipose tissue engineering based on human preadipocytes combined with gelatin microspheres containing basic fibroblast growth factor. Biomaterials 24: 2513–2521.
  20. Lu, F., H. Mizuno, A.C. Uysal, X. Cai, R. Ogawa, H. Hyakusoku (in press) Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg.
  21. Matsumoto, D., K. Sato, K. Gonda, Y. Takaki, T. Shigeura, T. Sato, E. Aiba-Kojima, F. Iizuka, K. Inoue, H. Suga, K. Yoshimura (2006) Cell-assisted lipotransfer (CAL): supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng 12: 3375–3382.
  22. Mizuno, H., P.A. Zuk, M. Zhu, H.P. Lorenz, P. Benhaim, M.H. Hedrick (2002) Myogenic differentiation by human processed lipoaspirate cells. Plast Reconstr Surg 109: 199–209.
  23. Ogawa, R., H. Mizuno, A. Watanabe, M. Migita, M. Shimada, H. Hyakusoku (2004) Osteogenic and chondrogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice. Biochem Biophys Res Commun 313: 866–872.

    External Resources

  24. Okabe, M., M. Ikawa, K. Kominami, T. Nakanishi, Y. Nishimune (1997) ‘Green mice’ as a source of ubiquitous green cells. FEBS Lett 407: 313–319.
  25. Orr, T.E., A.M. Patel, B. Wong, G.P. Hatzigiannis, T. Minas, M. Spector (1999) Attachment of periosteal grafts to articular cartilage with fibrin sealant. J Biomed Mater Res 44: 308–313.
  26. Patrick, C.W., Jr. (2000) Adipose tissue engineering: the future of breast and soft tissue reconstruction following tumor resection. Semin Surg Oncol 19: 302–311.
  27. Patrick, C.W., Jr. (2001) Tissue engineering strategies for adipose tissue repair. Anat Rec 263: 361–366.
  28. Patrick, C.W., Jr., P.B. Chauvin, J. Hobley, G.P. Reece (1999) Preadipocyte seeded PLGA scaffolds for adipose tissue engineering. Tissue Eng 5: 139–151.
  29. Peer, L.A., J.C. Walker (1951) The behavior of autogneous human tissue grafts: II. Plast Reconstr Surg 7: 73–84.
  30. Perka, C., O. Schultz, R. Spitzer, K. Lindenhayn, G. Burmester, M. Sittinger (2000) Segmental bone repair by tissue-engineered periosteal cell transplants with bioresorbable fleece and fibrin scaffolds in rabbits. Biomaterials 21: 1145–1153.
  31. Pittenger, M.F., A.M. Mackay, S.C. Beck, R.K. Jaiswal, R. Douglas, J.D. Mosca, M.A. Moorman, P.W. Simonetti, S. Craig, D.R. Marshak (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284: 143–147.
  32. Planat-Benard, V., J.S. Silvestre, B. Cousin, M. Andre, M. Nibbelink, R. Tamarat, M. Clergue, C. Manneville, C. Saillan-Barreau, M. Duriez, A. Tedgui, B. Levy, L. Penicaud, L. Casteilla (2004) Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspective. Circulation 109: 656–663.
  33. Rehman, J., D. Traktuev, J. Li, S. Merfeld-Clauss, C.J. Temm-Grove, J.E. Bovenkerk, C.L. Pell, B.H. Johnstone, R.V. Considine, K.L. March (2004) Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation 109: 1292–1298.
  34. Silverman, R.P., L. Bonasser, D. Passeretti, M.A. Randolph, M.J. Yaremchuk (2000) Adhesion of tissue-engineered cartilage to native cartilage. Plast Reconstr Surg 105: 1393–1398.
  35. Tholpady, S.S., R. Schlosser, W. Spotnitz, R.C. Ogle, W.H. Lindsey (1999) Repair of an osseous facial critical-size defect using augmented fibrin sealant. Laryngoscope 109: 1585–1588.
  36. Wei, J.P., T.S. Zhang, S. Kawa, T. Aizawa, M. Ota, T. Akaike, K. Kato, I. Konishi, T. Nikaido (2003) Human amnion-isolated cells normalize blood glucose in streptozotocin-induced diabetic mice. Cell Transplant 12: 545–552.
  37. Yoshimura, H., T. Muneta, A. Nimura, A. Yokoyama, H. Koga, I. Sekiya (2007) Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle. Cell Tissue Res 327: 449–462.
  38. Young, F.E. (2000) A time for restraint. Science 287: 1424.
  39. Zuk, P.A., M. Zhu, H. Mizuno, J.I. Huang, J.W. Futrell, A.J. Katz, P. Benhaim, H.P. Lorenz, M.H. Hedrick (2001) Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng 7: 211–228.

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