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Vol. 28, No. 2, 2008
Issue release date: January 2008
Section title: Original Report: Laboratory Investigation
Am J Nephrol 2008;28:324–329
(DOI:10.1159/000111825)

A Comparative Evaluation of Various Methods for Microalbuminuria Screening

Sarafidis P.A. · Riehle J. · Bogojevic Z. · Basta E. · Chugh A. · Bakris G.L.
aHypertension/Clinical Research Center, Department of Preventive Medicine, Rush University Medical Center, and bHypertensive Diseases Unit, Section of Endocrinology, Diabetes and Metabolism, University of Chicago-Pritzker School of Medicine, Chicago, Ill., USA; cSection of Nephrology and Hypertension, 1st Department of Medicine, AHEPA University Hospital, Aristotle University of Thesaloniki, Thessaloniki, Greece

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Article / Publication Details

First-Page Preview
Abstract of Original Report: Laboratory Investigation

Received: 9/4/2007
Accepted: 10/16/2007
Published online: 11/29/2007

Number of Print Pages: 6
Number of Figures: 4
Number of Tables: 3

ISSN: 0250-8095 (Print)
eISSN: 1421-9670 (Online)

For additional information: http://www.karger.com/AJN

Abstract

Background/Aims: Microalbuminuria is a marker of abnormal vascular response and a predictor of cardiovascular morbidity and mortality. We evaluated a new quantitative office-based method to assess urinary albumin excretion (UAE) and compared it to other established methods. Methods: Spot urine samples from 165 patients were analyzed at a single study site using the HemoCue system, Clinitek Microalbumin, and Chemstrip Micral test, as well as at a central laboratory, where UAE and creatinine levels were measured. The central laboratory UAE values were used as reference. We evaluated the validity of the HemoCue results and compared them to the respective data for the laboratory albumin-to-creatinine ratio (ACR). Additionally, we assessed, diagnostic sensitivity, specificity, and positive and negative predictive values of all four methods, as well as the reproducibility of the HemoCue measurements. Results: Linear regression analysis demonstrated a good correlation for the HemoCue system (y = 0.9978x – 1.0217, R2 = 0.904) and ACR (y = 0.0815x + 0.3373, R2 = 0.784). Sensitivity and specificity for microalbuminuria diagnosis were 92 and 98% for HemoCue, 73 and 96% for ACR, 100 and 81% for Clinitek Microalbumin, and 70 and 83% for Chemstrip Micral dipstick, respectively. The correlation coefficient of duplicate HemoCue measurements was r = 0.98 (p < 0.001). Conclusions: The HemoCue system for microalbuminuria detection was as accurate and precise as laboratory ACR estimations. Its diagnostic performance was much better than that of widely used dipstick methods.


Article / Publication Details

First-Page Preview
Abstract of Original Report: Laboratory Investigation

Received: 9/4/2007
Accepted: 10/16/2007
Published online: 11/29/2007

Number of Print Pages: 6
Number of Figures: 4
Number of Tables: 3

ISSN: 0250-8095 (Print)
eISSN: 1421-9670 (Online)

For additional information: http://www.karger.com/AJN


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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

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    External Resources

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