A Single Oral Sensitization to Peanut without Adjuvant Leads to Anaphylaxis in MiceProust B. · Astier C. · Jacquenet S. · Ogier V. · Magueur E. · Roitel O. · Belcourt C. · Morisset M. · Moneret-Vautrin D.A. · Bihain B.E. · Kanny G.
aEA 3999 ‘Allergic Diseases: Diagnosis and Therapeutics’, Department of Internal Medicine, Clinical Immunology and Allergology, University Hospital, Central Hospital, Nancy, and bJE 2482 Lipidomix, Laboratory of Molecular Medicine and Therapeutics, Vandoeuvre-lès-Nancy, France
Background: A model of peanut food allergy has been developed in mice using a simple sensitization protocol leading to a quantitatively measurable allergic response. Methods: C3H/HeJ mice received a single intragastric administration of whole peanut (80 mg) without adjuvant. Two weeks later, intraperitoneal challenge with peanut extract led to a severe anaphylaxis. Results: Anaphylactic reaction was evidenced by vascular leakage, severe clinical symptoms, a drop in body temperature, a decrease in breathing rate and also by increased concentrations of serum mouse mast cell protease-1. Sensitization to peanut was demonstrated by positive skin tests (ear swelling test and intradermal skin testing) and increased peanut-specific IgE levels. Conclusions: Thus, we obtained a model of severe peanut hypersensitivity within 2 weeks following single oral exposure without adjuvant. This model may be useful for further basic and applied studies on peanut allergy.