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Vol. 35, No. 3, 2008
Issue release date: June 2008
Section title: Review Article · Übersichtsarbeit
Free Access
Transfus Med Hemother 2008;35:160–167
(DOI:10.1159/000124734)

Mesenchymal Progenitor Cells: Tissue Origin, Isolation And Culture

Bourin P.a · Gadelorge M.a · Peyrafitte J.b · Fleury-Cappellesso S.a · Gomez M.a · Rage C.a · Sensebe L.c
a Laboratoire d’Ingénierie cellulaire, GECSoM, Etablissement Français du Sang Pyrénées-Méditerranée, bUMR CNRS UPS 5241, Toulouse, c Laboratoire de recherche, GECSoM, Etablissement Français du Sang Centre-Atlantique, Tours, France
email Corresponding Author

Abstract

Since the pioneering work of Alexander Friedenstein on multipotent mesenchymal stromal cells (MSCs), a tremendous amount of work has been done to isolate, characterize and culture such cells. Assay of colony forming unit-fibroblasts (CFU-Fs), the hallmark of MSCs, is used to estimate their frequency in tissue. MSCs are adherent cells, so they are easy to isolate, and they show contact inhibition. Thus, several parameters must be taken into account for culture: cell density, number of passages, culture medium, and growth factors used. The purity of the initial material is not a limiting parameter. Similar but not identical cell populations are found in almost all mammal or human tissues. MSCs seem to be very abundant in adipose tissue but at low frequency in blood from umbilical cord or in adult tissue. The culture conditions are very similar, whatever the source of cells. Because of their favorable properties, MSCs are very promising tools for regenerative medicine.

© 2008 S. Karger AG, Basel


  

Key Words

  • Multipotent mesenchymal stromal cells
  • Tissue origin
  • Cell culture

Introduction


  

Author Contacts

Dr. Philippe Bourin, Laboratoire d’Ingénierie cellulaire, Etablissement Français du Sang Pyrénées-Méditerranée, 75 rue de Lisieux, 31300 Toulouse, France, Tel. +33 534 5024-78, Fax -70, E-mail philippe.bourin@efs.sante.fr

  

Article Information

Received: December 4, 2007
Accepted: February 14, 2008
Published online: May 8, 2008
Number of Print Pages : 8
Number of Figures : 2, Number of Tables : 0, Number of References : 78

  

Publication Details

Transfusion Medicine and Hemotherapy

Vol. 35, No. 3, Year 2008 (Cover Date: June 2008)

Journal Editor: Sibrowski W. (Münster)
ISSN: 1660–3796 (Print), eISSN: 1660–3818 (Online)

For additional information: http://www.karger.com/TMH


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Abstract

Since the pioneering work of Alexander Friedenstein on multipotent mesenchymal stromal cells (MSCs), a tremendous amount of work has been done to isolate, characterize and culture such cells. Assay of colony forming unit-fibroblasts (CFU-Fs), the hallmark of MSCs, is used to estimate their frequency in tissue. MSCs are adherent cells, so they are easy to isolate, and they show contact inhibition. Thus, several parameters must be taken into account for culture: cell density, number of passages, culture medium, and growth factors used. The purity of the initial material is not a limiting parameter. Similar but not identical cell populations are found in almost all mammal or human tissues. MSCs seem to be very abundant in adipose tissue but at low frequency in blood from umbilical cord or in adult tissue. The culture conditions are very similar, whatever the source of cells. Because of their favorable properties, MSCs are very promising tools for regenerative medicine.

© 2008 S. Karger AG, Basel


  

Author Contacts

Dr. Philippe Bourin, Laboratoire d’Ingénierie cellulaire, Etablissement Français du Sang Pyrénées-Méditerranée, 75 rue de Lisieux, 31300 Toulouse, France, Tel. +33 534 5024-78, Fax -70, E-mail philippe.bourin@efs.sante.fr

  

Article Information

Received: December 4, 2007
Accepted: February 14, 2008
Published online: May 8, 2008
Number of Print Pages : 8
Number of Figures : 2, Number of Tables : 0, Number of References : 78

  

Publication Details

Transfusion Medicine and Hemotherapy

Vol. 35, No. 3, Year 2008 (Cover Date: June 2008)

Journal Editor: Sibrowski W. (Münster)
ISSN: 1660–3796 (Print), eISSN: 1660–3818 (Online)

For additional information: http://www.karger.com/TMH


Article / Publication Details

First-Page Preview
Abstract of Review Article · Übersichtsarbeit

Received: 12/4/2007
Accepted: 2/14/2008
Published online: 5/8/2008
Issue release date: June 2008

Number of Print Pages: 8
Number of Figures: 2
Number of Tables: 0

ISSN: 1660-3796 (Print)
eISSN: 1660-3818 (Online)

For additional information: http://www.karger.com/TMH


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.